Hi, I was testing for Siderophore producing bacteria but I found in one culture plate only a portion of my culture plate become pink in colour. What is the reason behind that ?
Development of orange to pink coloration on CAS plate, which is what I believe you adopted is positive for siderophore production. The orange colour I am seeing to my right is suggestive of substance that made presence where and when it was not needed. If you claim there was no contamination, then you don't have a problem. This is why reproducibility of research is most important. It will remove chance events from real life situations under same or similar conditions.Repeat the assay two to three times and see what happens. If it recurs, then analyze the affected area using the "eye of the organic chemist" - the Thin layer chromatography.
I agree with Maurice Ekpenyong' s answer, it could be some transparent bacterial strain or some other contaminants in your CAS medium. Results of CAS test are mostly in the form of circular zone around the bacterial colony. I think, plate assay should always be performed in triplicate.
Hello the pink zone may be due to a contaminant. You need to culture the pink colored area and also the portion of colony overlapped by pink coloration
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