What is the recommended/suitable concentration of fungal spores for inoculation in biochar?

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What is the best procedure for phosphorus extraction from biochar by organic acids?

We are planning to extract phosphorus from biochar by organic acids. If anyone has some procedure (concentration of organic acids & steps) please inform.

01 February 2020 3,593 2 View

What is the recommended dose of biochar on microbial culture media?

We are going to use biochar on microbial culture media. At this situation, we are searching for the optimum dose (ammount of biochar in 1 lit of microbial culture media). Please inform if anyone...

07 August 2019 1,805 5 View

Is this a indication of Siderophore producing bacteria?

Hi, I was testing for Siderophore producing bacteria but I found in one culture plate only a portion of my culture plate become pink in colour. What is the reason behind that ?

06 July 2019 7,262 9 View

Is biochar effective for enhancing micro-nutrients to agricultural soil?

I found that biochar has a little impact for enhancement of micro-nutrients on agricultural soil. So, is there any information about the release of micro-nutrients from biochar to soil? Any...

04 May 2019 1,343 6 View

Detection of Indole acetic acid (IAA) production by actinobacteria. Please kindly advice me about the best method.

We are detecting Indole acetic acid (IAA) producing actinobacteria according to Bano and Musarrat (2003) method. The summary of the method is:- Inoculation of the isolates in LB medium...

01 January 1970 6,694 4 View

Iron phosphate instead of Tricalcium phosphate during pikovskaya media preparation.

Is it will be correct if I put Iron phosphate instead of Tricalcium phosphate during pikovskaya media preparation, for the determination of Iron phosphate solubilizing bacteria?

01 January 1970 7,171 6 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

How can I find access to heavy metal reference doses from the EPA and WHO websites?

Reference dose and Maximum acceptable concentrations HMs

03 August 2024 8,230 4 View

What is fitofthora instance of potatoes?

Now research fellow used it to prevent fungus in potato,may be potatoes gene effect.

02 August 2024 180 3 View

Should I remove an item from a scale to raise Cronbach's alpha and McDonald's omega or is it better to leave it if they are both over .7 already?

Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...

01 August 2024 4,606 7 View

Why 3 replicates for most biological assays? Is it enough to examine the data fits normal distribution?

Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...

31 July 2024 8,141 13 View

What is gangrine? Is there gangrine happened indie potato?

Please discuss someone told about fungus and allied others.

31 July 2024 3,372 3 View