We are going to use biochar on microbial culture media. At this situation, we are searching for the optimum dose (ammount of biochar in 1 lit of microbial culture media). Please inform if anyone have some idea about it. Thank you.
Dear Chandra,
I modestly assume that the threshold levels of biochar, which you are looking for, could widely vary based on the organism chosen, and on the basis of the biomass source/biochar composition. In the recent past, I have observed some articles stating both beneficial and detrimental impacts of biochar application to the soil microbial communities. I am providing you with the title of a recent article where the authors have determined the threshold levels of varying compositions of biochar on various microbes. It maybe of help to you.
Strain-Specific Effects of Biochar and Its Water-Soluble Compounds on Bacterial Growth
that is depends on the biochar composition and which microbe you are choose.
I agree with answers above. Basically the dose depends upon purpose why biochar is used. For example de Oliveira Mendes et al. (2014), Appl.Environ.Microbiol. 80(10):3081-3085 employed biochar concentration of 3 g.L-1 in culture medium to enhance rock phosphate solubilization by Aspergillus niger.
Best regards
Vit
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