What is the best etching method for Mg-Ca alloy?

I have used Picral etchant (4.2 g Picric acid, 10 mL Acetic acid, 70 mL Ethanol, 10 mL distilled water) but it didn't work. Is there any alternative?

21 February 2021 2,558 5 View

Which analysis method in XRD analysis is the most accurate way to measure crystallite size & lattice starin? Scherrer, Williamson-Hall or Rietveld?

Here are three different ways to calculate crystallite size and lattice strain of a sample, based on XRD peaks: "Scherrer" calculation, "Williamson-Hall" or "Rietveld". Each calculation is proper...

04 January 2021 7,836 4 View

How can I produce cell line from human primary fibroblast cells, extracted from synovial Tissue?

I am working on human fibroblast cells from synovial tissue and now they are in 4-5 passages. These cells have been confirmed with specific markers (99% purity). Recently, I have decided to...

17 November 2020 627 3 View

How can I fix this error in HFSS software?

HFSSDesign1 (DrivenModal) [warning] Com Engine non-responsive since 01:04:19, June 08, 2020. Can be due to CPU intensive processing or network problems. If persisting for long, manually...

07 June 2020 2,376 8 View

How can I find about highly cited and hot paper by my affiliation of Nutrition and Food Sciences research center in Scopus site?

How can I find about highly cited and hot paper by my affiliation of Nutrition and Food Sciences research center in Scopus site? I should search and find the number of citation since 3 years ago...

07 January 2020 3,776 10 View

What can people do to reduce personal health risks from air pollution ?

Tehran, the capital city of Iran, suffers from severe air pollution, and it is located near three major fault lines, while being the most populous region of the country. The officials have created...

23 December 2019 7,879 18 View

Is there any affinity between the gold nanoparticles and cellulose acetate filter?

I tried to filter the gold nanoparticle with cellulose acetate, 0.45 um filter. The hydrodynamic size of my nanoparticle are about 100nm (you can see the DLS analysis of them). The gold...

02 October 2019 7,224 7 View

Would you please suggest a suitable and uptodate reference to check the volume of distribution of each drug?

I want to have the volume of distribution of vancomycin but I found variable amounts between 0.5, 0.7, and 0.9 L/Kg in different references. What is the best value in critically ill patients? And...

15 August 2019 5,955 3 View

What do yo think about " Role of Perceptions of Prejudice and Discrimination on the Adjustment of articles in some journals"?

Do you think in few journals you have seen " The Role of Perceptions of Prejudice and Discrimination on the Adjustment?

07 August 2019 4,486 32 View

What are the main reasons for being aggressive in jobs or relationships?

The number of angry people around us are increasing every day and aggressive behavior ....... why?

27 June 2019 1,800 38 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

FDC and FRC cell populations in mouse spleen?

I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...

17 February 2021 3,506 4 View

What to do with absence of fluorescence label in Fucci2a mouse-derived fibroblast culture?

Dear all, I have harvested fibroblast cells from R26p-Fucci2 mouse embryos (mouse line as described in this paper: https://dev.biologists.org/content/140/1/237) and cultured them on plastic,...

17 February 2021 3,368 2 View

Is there a way in which microglia can be extracted from mice without killing them ?

I'm aiming to use an adoptive cell transfer approach, with microglia in mice with ALS. So far all the protocols I have seen, require sacrificing the mouse. I'd like to re-infuse the microglia back...

11 February 2021 9,509 1 View

Does euthanasia by CO2 inhalation cause NF-kB activation in mouse heart or body in general?

I was wondering if euthanizing mice by carbon dioxide inhalation would cause activation of NF-kB inflammatory response?

26 January 2021 4,096 2 View

[Covid-19] GGO Evaluation - CT imaging of the lungs ?

13 January 2021 3,115 4 View

Why do my fibroblasts under hypoxia conditions proliferate slower than the same fibroblasts under normoxic conditions?

I am using healthy control lung fibroblasts in a 96 hour experiment with two drugs (nintedanib and Pirfenidone) and vehicle controls for both. I have both hypoxia (approx 1% O2) and normoxia...

12 January 2021 6,260 1 View

Can I measure lung weight after formalin fixation?

Hi, I have simple quick question. Can I make a measurement of mouse lung to evaluate lung metastasis after formalin fixation? Is it not reliable? Thanks.

11 January 2021 3,178 3 View

Does someone had trouble with fixable life/dead in endometrial flow cytometry?

Hi. I am doing flow cytometry of endometrial cells (read epithelial and fibroblast). The antibodies I need to use are both intracellular. In the same time I want to get only life cells. Thus I...

07 January 2021 6,473 3 View