I'm looking for the specific marker of lung fibroblast in systemic sclerosis.
I am conducting a systematic review and meta analysis; as I am extracting data I realized there is a consort diagram that shows the number of patients from randomization till end of the study. So...
01 August 2024 9,993 3 View
I have a mixture of glycol, water, MDEA, and piperazine. I want to analyze it with GC-FID.
15 July 2024 9,652 3 View
I have a mixture of glycol, water, MDEA, and piperazine which I want to analyze with GC.
15 July 2024 2,181 10 View
Hello I want to know is there any way to download all 3d structures of natural products of IMPPAT database?
14 June 2024 7,298 1 View
We prepared a PCR buffer and assessed it with approximately 12 primer pairs, all of which resulted in successful amplification. However, there was a specific gene region that failed to amplify....
13 June 2024 548 3 View
when I insert my primers in primer blast site and push the button "get primers" I just reach different errors and I don't know what the problem is!
22 April 2024 8,001 5 View
I want to culture Huvecs cells under hypoxic condition (hypoxic chamber), Does anyone know the cell culture and treatment process?
15 February 2024 6,732 0 View
Hello! I inject 1 mg/ml Collagen + matrigel mixture in one channel of my device and 2mg/ml collagen in the adjacent channel. Two channels are separated by microposts. I somehow see hydrogel...
11 February 2024 8,181 1 View
Dear researchers, I am seeking assistance in synthesizing ZIF-67 for my laboratory-based PhD thesis. Have any of you successfully synthesized ZIF-67 using any of the following methods: 1)...
18 December 2023 8,152 1 View
Hello! I wanted to buy mCherry SUM159. Our supplier won't work anymore and I couldn't find any websites to order it. Would you please let me know if you know someone to help me with that? Thank you
24 October 2023 881 0 View
I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...
29 July 2024 3,030 2 View
hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...
21 July 2024 8,660 1 View
I am using fibroblast growth factor, purchased in powder form. What suitable substance should be added to get it in a liquid form? Thanks
03 July 2024 9,866 2 View
Hello, everyone. I would like ask one question. I transfected fibroblasts with EF1A-myod-puro using lipofectamine 3000. Promoter for puro is CMV. After transfection 48 hours, I did puromycin...
23 June 2024 4,902 3 View
It is known that patients with desminopathy often die from pneumonia. Have pathomorphological studies of the lungs been performed in patients with desminopathy?
15 June 2024 1,355 7 View
Bfgf was aliquoted in media and transported at 22 degee Celsius. How long it is stable
15 June 2024 3,010 2 View
Hi everyone, I am working with an adhesive fibroblast cell line in roller flasks (Cell Master Bottles). When culturing the cells with FBS that is heated with FBS (around 60 degrees C), the cells...
02 June 2024 7,409 1 View
I want to compare the RDW index between 2 groups of patients with stable COPD and COPD in acute exacerbations, so I calculated the sample size using the formula to estimate a ratio or compare 2...
02 June 2024 2,994 1 View
I started a cell culture with a mix of suspended and adherent cells (presumably fibroblasts) on May 10th. After a week, I treated them for adherent cells only. On May 24th, most of the adherent...
02 June 2024 4,903 5 View
Recently I've been doing some indirect co-culture experiments, in a nutshell, treating cancer cells with CAF's conditioned medium. So how do you do the collection of conditioned media and the...
01 June 2024 9,484 2 View
