Hi

in the tools at UCSC website, there is "in silica PCR" that could easily replace it.

and for information, lot of other tools could interest you.

all the best

fred

Depending on the error, you have to adjust your parameters.

Dear Parisa,

You can just run Nucleotide BLAST in NCBI with Primer sequence in FASTA as a query. It will show you the similarity match with the targets with % identity, Query coverage, and Alignment with the target.

From all this information you can easily identify the binding position as well as specificity of the designed or selected primers.

Hope it will help you.

Thanks- Ananya

Dear Ananya

Thanks for your help

Best

Parisa

Dear Parisa,

You're most welcome.

Best wishes.

Ananya