Dear all researchers,

I would like to know whether is there any possibility of changes in spectrum of GFP-positive cells in vitro and after implantation (in which can be done by spectral analysis using confocal microscopy)? In other words, the cells altered the spectrum of GFP.

Based on my reading (might need more search), less study analyse the spectral emission of cultured GFP-positive cells before implantation which is aim to confirm the identity and behaviour of GFP-positive cells. Most studies do transduction efficiency test via flow cytometry (in case if you do lentiviral GFP transduction not using cells from transgenic animal). However, these two conditions are different: transduction efficiency and; spectrum emission of GFP before and after implantation in which latter condition can confirm the behaviour and identity of GFP-cells.

Thank you very much for your response.

Warm regards,

Nisa