Dear all.,

I'm analyzing myoglobin content in ground fresh meat that has been mixed with red pigment solution using UV spectrophotometer at 503, 525, 557, 572, 582, and 700 nm. My red pigment has a maximum absorption maximum at around 460.2, 490.8, 520.4, and 360.6 nm. May I know if those pigment solution readings will interfere with the myoglobin readings? I did the colour blank to minus with the myoglobin readings but the blank colour has high readings value as compared to myoglobin readings (maybe due to water loss activity in fresh meat). Can I just ignore the pigment solution readings? Thank you very much for your reply.

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