Khairunnisa Ramli

4 Questions 23 Answers 0 Followers

Questions related from Khairunnisa Ramli

Is there anyone had observed changes in GFP spectrum of GFP-positive cells from harvested tissue?

Dear all researchers, I would like to know whether is there any possibility of changes in spectrum of GFP-positive cells in vitro and after implantation (in which can be done by spectral analysis...

13 December 2016 8,785 3 View

Is there any alternative for antigen retrieval method in case of frozen section?

Dear all researchers, I am a beginner in immunohistochemical staining. I did frozen section for my fixed human and rat nerve for optimization of antibody. I did tried with heat induced antigen...

30 March 2016 1,638 22 View

What is the best protocol to do immunohistochemical analysis on GFP-positive tissue section?

Dear researchers. I did IHC on GFP-positive tissue section with heat antigen retrieval step, followed by normal protocol. Finally, I didnt managed to view any fluorescence from GFP as viewed...

23 July 2015 6,881 5 View

Has anyone experienced the effect of fixing a nerve sample in 4% PFA instead of 4% GA for TEM analysis?

Hi, I'm interested to know the effect on myelin sheath of myelinated nerve fibers after fixation in 4% PFA? is there any difference if we fix in 4% GA (glutaraldehyde). Thanks for your response....

18 June 2015 1,176 3 View