I am studying arsenic biotransformation by bacteria and I need to check the morphological changes on the bacterial cells after exposure to arsenic. I also need to check if there's any arsenic accumulation or adsorption in the bacterial cell. How can I best prepare my sample for this purpose? We don't have critical point drying machine here so is there any other alternative to this? Thank you.
Hello if you have controll culture wich not used for Arsenic consumption just use same amount of biomass and make digestion in nitric acid, better to perform microwave digestion and measure Arsenic content by any available technic AAS GFA or ICP OES or MS. After that you can go with your FESEM.
Karen A. Darbinyan wouldn't digestion in HNO3 affect the structural integrity of the bacterial cell? I need the cell to remain intact for the purpose I stated above.
First better to detect quantitatively any accumulation of Arsenic than perform analysis. For detection you need to use about 20-80mg of cells digestion in HNO3.
Karen A. Darbinyan thank you for your valuable input sir. for cell digestion in HNO3, do I use dry or wet biomass?
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