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Hi everyone, I am recently overepressing mouse ULK2 in HEK-293T cells. However, the expression level is much lower than other genes constructed with the same vector. So, is there any methods to...
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Hi everyone, Recently, I am performing IP using anti-Flag Ab(M2, F3165) for Flag-ULK1 overexpression cells. But the efficiency is very low. Even if the input accounts for 1% of the whole cell...
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Hi, Everyone are looking for the protein target of a small molecule, but is there any possibility that a small molecule target another small molecules? Thanks a lot in advance! Zhiyuan
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Hi everyone, Searching the target of a specific small molecule is difficult. Could anyone offer some good advice or suggestions for strategy to find the target of a small molecule. Thanks a lot in...
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Hi everyone, I am recently performing RNAi by Tet-Inducible shRNA system. Although 2ug/ml doxycline induction for 72 hours could induce the fluorescent tag RFP expression obviously, the knockdown...
10 December 2015 5,270 4 View
Hi everyone, Endosome exist as two forms:vesicle and tubule. And the two forms are in a dynamic balance. So who can tell me what is mechanism underlying the the two forms transition?...
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Recently I am performing the transfection assay using Fugene 6 reagent in HEK 293T cell. The plasmid includes the EGFP gene. Even I use the vector including only GFP without the fusion gene, the...
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