Zhiyuan li

9 Questions 18 Answers 0 Followers

Questions related from Zhiyuan li

Is there any methods to improve mouse ULK2 expression and transfection efficiency?

Hi everyone, I am recently overepressing mouse ULK2 in HEK-293T cells. However, the expression level is much lower than other genes constructed with the same vector. So, is there any methods to...

15 June 2018 8,305 2 View

Why the efficiency of Flag antibody IP is very low?

Hi everyone, Recently, I am performing IP using anti-Flag Ab(M2, F3165) for Flag-ULK1 overexpression cells. But the efficiency is very low. Even if the input accounts for 1% of the whole cell...

26 August 2016 4,718 4 View

Does anyone have some recommadations for coverslips used in 24-well plate?

Hi everyone, I am recently searching coverslips used in 24-well plate due to the fact that the coverslips we used (12-545-81,fisherbrand) is in import prohibition condition. So, does anyone could...

13 April 2016 4,246 0 View

Could a small molecule target another small molecules?

Hi, Everyone are looking for the protein target of a small molecule, but is there any possibility that a small molecule target another small molecules? Thanks a lot in advance! Zhiyuan

01 April 2016 5,823 4 View

How to find the target of a small molecule?

Hi everyone, Searching the target of a specific small molecule is difficult. Could anyone offer some good advice or suggestions for strategy to find the target of a small molecule. Thanks a lot in...

01 April 2016 4,985 5 View

Why doesn't Tet-Inducible shRNA work well?

Hi everyone, I am recently performing RNAi by Tet-Inducible shRNA system. Although 2ug/ml doxycline induction for 72 hours could induce the fluorescent tag RFP expression obviously, the knockdown...

10 December 2015 5,297 4 View

Does anyone know anything (protein, lipid etc) that could make endosome tubulation?

Hi everyone, Endosome exist as two forms:vesicle and tubule. And the two forms are in a dynamic balance. So who can tell me what is mechanism underlying the the two forms transition?...

13 June 2015 6,539 3 View

Has anyone performed cAMP glo to detect the Dopamine receptor D3 response to dopamine?

Hi everyone, I am recently performing the cAMP Glo assay（promega kit）to detect dopamine receptor D3 response to the ligand dopamine in HeLa and CHO cells.I plated 5000 cells in 384-well plates on...

27 October 2014 7,791 5 View

Fugene 6 transfection troubleshooting in HEK 293T

Recently I am performing the transfection assay using Fugene 6 reagent in HEK 293T cell. The plasmid includes the EGFP gene. Even I use the vector including only GFP without the fusion gene, the...

06 September 2014 5,018 6 View