Zhiyuan li

6 Questions 12 Answers 0 Followers

Questions related from Zhiyuan li

Is there any methods to improve mouse ULK2 expression and transfection efficiency?

Hi everyone, I am recently overepressing mouse ULK2 in HEK-293T cells. However, the expression level is much lower than other genes constructed with the same vector. So, is there any methods to...

15 June 2018 8,277 2 View

Why the efficiency of Flag antibody IP is very low?

Hi everyone, Recently, I am performing IP using anti-Flag Ab(M2, F3165) for Flag-ULK1 overexpression cells. But the efficiency is very low. Even if the input accounts for 1% of the whole cell...

26 August 2016 4,630 4 View

Could a small molecule target another small molecules?

Hi, Everyone are looking for the protein target of a small molecule, but is there any possibility that a small molecule target another small molecules? Thanks a lot in advance! Zhiyuan

01 April 2016 5,797 4 View

Why doesn't Tet-Inducible shRNA work well?

Hi everyone, I am recently performing RNAi by Tet-Inducible shRNA system. Although 2ug/ml doxycline induction for 72 hours could induce the fluorescent tag RFP expression obviously, the knockdown...

10 December 2015 5,268 4 View

Does anyone know anything (protein, lipid etc) that could make endosome tubulation?

Hi everyone, Endosome exist as two forms:vesicle and tubule. And the two forms are in a dynamic balance. So who can tell me what is mechanism underlying the the two forms transition?...

13 June 2015 6,507 3 View

Fugene 6 transfection troubleshooting in HEK 293T

Recently I am performing the transfection assay using Fugene 6 reagent in HEK 293T cell. The plasmid includes the EGFP gene. Even I use the vector including only GFP without the fusion gene, the...

06 September 2014 4,989 6 View