Why do we wash azolectin with acetone?

I want to prepare liposomes made of azolectin (sometimes spelled as asolectin). I start with the azolectin powder, and many protocols say that I should wash it with acetone (dissolve, centrifuge,...

04 February 2021 2,738 3 View

How to solve 1-D Wave equation?

Good day, In standard PDE literature, there are a lot of solution approaches for the 1-D wave equation initial-boundary value problem. One way is with separation of variables. Below I describe...

25 January 2021 4,876 3 View

Journal Quartiles for Research Evaluation?

Dear all, Could you please tell me the countries where the journal quartiles SJR/JCR are used for the state system for research evaluation (except for Russia)? Best, Dmitry

21 June 2020 5,635 7 View

Could you please suggest an XRD data viewer?

Hi. I'd appreciate if someone could suggest a powder XRD (NPD, PDF as an option) data viewer which: (1) Is free of charge (2) Is easy to operate, i.e. can be run with a double-click, multiple...

06 June 2020 6,493 2 View

Could my SP2/0-Ag14 be cross-contaminated?

Hello! Currently I propagating my SP2/0 for hybridoma. It's a first time when I working with this line. According to ATCC this line must grow in suspension, but when I'm doing my daily observation...

20 March 2020 5,191 2 View

How we can painting with powder by electrostatic method without Faraday effect?

Good day, dear colleagues I have some question for You I'm working on the powder painting of details with difficult geometry. The tribostatic method has a good result about this I try to use...

25 February 2020 2,190 3 View

Manuscripts for the 4th volume of the "Biodiversity, biogeography and nature conservation in Wallacea and New Guinea"?

26 November 2019 1,452 4 View

Where to go to the conference?

20 October 2019 6,938 3 View

Can I leave slides in Ventana overnight after finishing of staining program?

Hello everyone! On my previous job I worked with Ventana Benchmark Ultra and performed various IHC stainigs on this device. As far as I can remember, engineer said, that after finishing of...

21 February 2019 3,193 2 View

How to get correct information on the current level of development of stem cell medicine?

Stem cell research is a long-standing subject. Quite a lot is known for stem cells. But their massive use in medicine is a novelty of recent times. This area of medicine is developing rapidly....

14 January 2019 9,853 1 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View