Hi friends,

I am extracting Pr content of Arabidopsis thaliana leaves by TCA acetone method to prepare them for MS spectrometer analysis (by use of in gel digestion protocole). For this, I am supposed to resolve pellet of final step in 1x SDS sample buffer (containing SDS, glycerol, B mercaptoethanol and bromo phenol blue). The problem is that I would not be able to determine Pr concentration by Lowry, Bradford method as well as by Qubit kit before sending samples for MS analysis. So, I wanted to ask if you know any other solution for resolving pellet to determine pr concentration before using sample buffer? Or even is there any other way I can do to determine pr concentration accurately after resolving pellet in SDS sample buffer?  

Thanks in advance,