Zahra Mirzahosseini

4 Questions 8 Answers 0 Followers

Questions related from Zahra Mirzahosseini

Do you know what is the threshold for up and down-regulation in proteomics?

Hi friends, After doing LC-MS MS what threshold fold change do researchers usually consider for up and down regulation? I mean, what is the significant fold change? In some paper 1.6 fold change...

16 July 2018 1,127 9 View

Do you know a bioinformatic software or website by which you can classify the whole proteom of Arabidopsis thaliana?

Hi friends, As a result of proteomics (LC MS/MS) of Arabidopsis thaliana leaves, I have more than 1000 identified Proteins. But I just need to work with some groups (for example defensive...

18 December 2017 8,058 5 View

Does anyone know a protein or peptide using as control in SDS PAGE?

Hi friends, I am looking for a protein in plants (Arabidopsis in my case) that remain unchanged in different conditions or treatments to use as a reference pr in SDS PAGE. Similar to real time...

08 December 2017 2,410 3 View

Is there any solution to resolve pellet in TCA acetone method?

Hi friends, I am extracting Pr content of Arabidopsis thaliana leaves by TCA acetone method to prepare them for MS spectrometer analysis (by use of in gel digestion protocole). For this, I am...

24 April 2017 6,385 3 View