...
Which one of the following terms are the best and more accurate: SAGE (serial analysis gene expression) or microarray? Regards.
01 February 2014 6,932 1 View
Are there method(s), algorithm(s) , and software that can determine the activity site and the function of genes and proteins.
31 December 2013 786 4 View
The following are sequences of Staphylokinase gene of Staphylococcus aureus bacteria How can I amplified the gene with these primers using NCBI (in silico) Forward primer: 5' -...
11 December 2013 5,440 4 View
Are there any programs or software's that I can use?
11 December 2013 6,868 2 View
What is the difference(s) between phylogenetic method and cluster (hierarchical) analysis? Which one is more accurate?
07 August 2013 2,532 0 View
How can I get some real data of microarray with examples and more details? Thanks in advance.
06 July 2013 7,042 2 View
I'm Interested in gene expression and I want to know how calculate the fold difference. The reference I read is: Microarray Bioinformatics, Stekel Dov , 2003, Cambridge university, pp 116. How can...
01 February 2013 9,429 0 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
Molecular docking software/ websites?
02 August 2024 8,704 7 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...
28 July 2024 2,979 1 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
