SAGE and Microarray?

Which one of the following terms are the best and more accurate: SAGE (serial analysis gene expression) or microarray? Regards.

01 February 2014 6,874 1 View

What is the activity site and the function of genes and proteins?

Are there method(s), algorithm(s) , and software that can determine the activity site and the function of genes and proteins.

31 December 2013 727 4 View

Any suggestions for amplifying genes with NCBI?

The following are sequences of Staphylokinase gene of Staphylococcus aureus bacteria How can I amplified the gene with these primers using NCBI (in silico) Forward primer: 5' -...

11 December 2013 5,374 4 View

How can I measure or determine the age of the species when I use a constructed phylogenetics tree?

Are there any programs or software's that I can use?

11 December 2013 6,807 2 View

The difference(s) between phylogenetic method and cluster analysis - hierarchical?

What is the difference(s) between phylogenetic method and cluster (hierarchical) analysis? Which one is more accurate?

07 August 2013 2,471 0 View

Real data of microarray?

How can I get some real data of microarray with examples and more details? Thanks in advance.

06 July 2013 6,970 2 View

Is there any software that can determine the function of genes and proteins in complete genomes?

...

05 June 2013 2,800 0 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

Which CpGs have a significant effect on gene expression?

It is known that methylated CpG around TSS decreases gene expression. However, It is difficult to know exactly which CpG has a greater impact on gene expression. Are there any databases known...

20 February 2021 5,634 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

Found that there was a multiple band when amplifiying targeted gene ?

i few days ago amplified specific gene using PCR with specific primers, but there was a multiple band in electrophoregram i found. out of 10 sample amplified, there was multiple band and there was...

18 February 2021 5,131 5 View