I'm Interested in gene expression and I want to know how calculate the fold difference.
The reference I read is: Microarray Bioinformatics, Stekel Dov , 2003, Cambridge university, pp 116. How can I get more real data about this subject?
Which one of the following terms are the best and more accurate: SAGE (serial analysis gene expression) or microarray? Regards.
01 February 2014 6,932 1 View
Are there method(s), algorithm(s) , and software that can determine the activity site and the function of genes and proteins.
31 December 2013 786 4 View
The following are sequences of Staphylokinase gene of Staphylococcus aureus bacteria How can I amplified the gene with these primers using NCBI (in silico) Forward primer: 5' -...
11 December 2013 5,440 4 View
Are there any programs or software's that I can use?
11 December 2013 6,868 2 View
What is the difference(s) between phylogenetic method and cluster (hierarchical) analysis? Which one is more accurate?
07 August 2013 2,532 0 View
How can I get some real data of microarray with examples and more details? Thanks in advance.
06 July 2013 7,042 2 View
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05 June 2013 2,858 0 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
Gene sequencing related trouble shooting
25 July 2024 4,149 2 View
I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?
23 July 2024 1,353 4 View
Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...
23 July 2024 2,673 6 View