I need a simple and inexpensive method -perhaps a biochemical marker- to measure oxygen free radical activity, or the damage caused by oxygen free radicals in blood. I can't seem to come up with anything. Any help would be appreciated.
I hope this article will can help you to measure the damage caused by oxygen free radicals .
ROS in blood have a very short half life. Might be better to measure the anti-oxidant capacity of plasma or blood, adding an oxidant (e.g. chloramine-T) to plasma and checking after 2 min how much oxidant can still be detected, either by 4 mol/l KJ incubation (405 nm absorbance) or by incubation with 0.3 mmol/l (final conc.) luminol and measuring the luminescence in a microtiter plate luminometer (e.g. LUMO).
It is to hard to measure oxigene free radical activity in blood. You have to measure Total Anioxidant Status (TAS) and Total Oksidant statüs (TOS). There are a lot of kits for TAS and TOS. And you can also calculate Oxidative Stress İndex by using this formül (OSI=TOS/TAS X %100)..
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