I am wondering if Trypsin is hard to use in immunoassays because of antibodies? Is Trypsinogen form easier to use in immunoassays? I need research results, like papers or reviews.
Remember that trypsinogen unless you have an inhibitor is easily degraded to trypsin, so most of the time you use trypsinogen, it is possible that trypsin also coexists.
In my case I used trypsinogen as an immunogen and the Mabs obtained recognized both trypsinogen and trypsin.
Hi Mustafa, trypsinogen is the precursor of trypsin (zymogen).
Trypsinogen by itself has almost no activity, but small amounts of active trypsin can generate a chain reaction that gives you a solution of fully active trypsin.
Thank you for your advices. Actually I am seeking for some strong articles, because i am currently writing a thesis and looking for good references. Papers i've found are so related but are not clearly suggests that " Trypsin is hard to use in immunoassays because it cuts antibodies.". I am searching pubmed 7/25 but still could not found something perfectly fits.
Here are a couple of papers that say that Ab is removed from cell surfaces by trypsin.
http://cancerres.aacrjournals.org/content/canres/52/4/835.full.pdf
http://www.bloodjournal.org/content/bloodjournal/84/4/1143.full.pdf?sso-checked=true
Will that do?
Hi Steingrimur, they look perfect.
I've just found some articles also, I will read papers you suggest and if they fit i will use them.
Thank you so much.
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