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Questions related from Mustafa Divyapıcıgil
Or should I use RNA polymerase and Reverse transcriptase to make RNA Spinach aptamers?
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I am wondering if Trypsin is hard to use in immunoassays because of antibodies? Is Trypsinogen form easier to use in immunoassays? I need research results, like papers or reviews.
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I am trying to find pI (isoelectric point) values of proteins with Bio-Rad's Rotofor. But i could not optimizing it, i am using Human Transferrin and its pI value is between 5.2 to 5.9 as we known...
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How can i elute His-Tagged proteins via using ELISA instead of IMAC? If your suggestion would be pH alteration, would it be a trustable way?
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I need a free web based or windows software based bioinformatics tool that can design an antibody against given antigen sequence. I have found Abie Pro 3.0 but i could not understood how to use it.
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