Mustafa Divyapıcıgil

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Questions related from Mustafa Divyapıcıgil

Can I design a DNA Spinach aptamer instead of RNA?

Or should I use RNA polymerase and Reverse transcriptase to make RNA Spinach aptamers?

11 November 2019 5,918 1 View

Is there any research about Trypsin restriction of antibodies?

I am wondering if Trypsin is hard to use in immunoassays because of antibodies? Is Trypsinogen form easier to use in immunoassays? I need research results, like papers or reviews.

07 July 2018 8,135 5 View

I need an advice about Rotofor optimization, can anybody help me?

I am trying to find pI (isoelectric point) values of proteins with Bio-Rad's Rotofor. But i could not optimizing it, i am using Human Transferrin and its pI value is between 5.2 to 5.9 as we known...

11 November 2016 9,220 9 View

How can i elute His-Tagged proteins via using ELISA?

How can i elute His-Tagged proteins via using ELISA instead of IMAC? If your suggestion would be pH alteration, would it be a trustable way?

08 August 2016 7,153 10 View

I need a free bioinformatics tool that can design an antibody against given antigen sequence, can you help me?

I need a free web based or windows software based bioinformatics tool that can design an antibody against given antigen sequence. I have found Abie Pro 3.0 but i could not understood how to use it.

07 July 2016 1,293 3 View