Or should I use RNA polymerase and Reverse transcriptase to make RNA Spinach aptamers?
Yes, you can do it by DNA/RNA hybridization.
Article Split Spinach Aptamer for Highly Selective Recognition of DN...
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How can i elute His-Tagged proteins via using ELISA instead of IMAC? If your suggestion would be pH alteration, would it be a trustable way?
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Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?
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