I need a free web based or windows software based bioinformatics tool that can design an antibody against given antigen sequence. I have found Abie Pro 3.0 but i could not understood how to use it.
If it were so simple, everybody would be doing it.
---
http://peds.oxfordjournals.org/content/25/10/507.abstract
Dear Mustafa,
Try using https://helixweb.nih.gov/AbDesigner/
However, it's limited to a few model organisms.
You may also try http://sysbio.chula.ac.th/AbDesigner3D/
Don't forget to cite.
Thank you so much Raza Ali Jafri
Or should I use RNA polymerase and Reverse transcriptase to make RNA Spinach aptamers?
10 November 2019 5,937 1 View
I am wondering if Trypsin is hard to use in immunoassays because of antibodies? Is Trypsinogen form easier to use in immunoassays? I need research results, like papers or reviews.
06 July 2018 8,157 5 View
I am trying to find pI (isoelectric point) values of proteins with Bio-Rad's Rotofor. But i could not optimizing it, i am using Human Transferrin and its pI value is between 5.2 to 5.9 as we known...
10 November 2016 9,243 9 View
How can i elute His-Tagged proteins via using ELISA instead of IMAC? If your suggestion would be pH alteration, would it be a trustable way?
07 August 2016 7,171 10 View
"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....
10 August 2024 8,621 1 View
Hello everyone, I am currently developing a thesis proposal and would appreciate your input on its viability and how to effectively carry it out. My proposed topic is: "Does the perceived threat...
10 August 2024 8,992 0 View
Who will bear moral responsibility for the deaths of thousands of people in the event of an earthquake? Weeks and months remain before the onset of strong earthquakes that bring death to...
08 August 2024 6,134 12 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...
06 August 2024 7,102 0 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks
06 August 2024 949 0 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View