As long as you have professional antigen presenting cells present in your stimulator population (either PBMC or splenocytes or activated DC culture), you can use soluble anti-CD3 antibodies + irradiated (or mitomycin-C treated) allogeneic feeder cells + low dose IL-2 to expand T-cells. If you're looking to expand CD4 cells or other, more specific, T-cell subsets you'd probably also need to add some more cytokines to your media to induce differentiation in the appropriate directions.

 Hi this paper give good directions

 J Exp Med. 1993 Jun 1;177(6):1643-50.

Contribution of direct and indirect recognition pathways to T cell alloreactivity.

Liu Z1, Sun YK, Xi YP, Maffei A, Reed E, Harris P, Suciu-Foca N.

Thanks Govinda Sharma and Paul E Harris. 

Actually, I did on mouse splenocytes from balb/c (Stimulator) and B6 (Responder) and it gave significant isotope counts for proliferation assay but the viable cells that I isolated from it were very very low (about 5% from initial total splenocytes). I did not use IL-2 because I followed the protocol of an old paper. This gives me a hint that it will be difficult to isolate allogeneic T cells from this culture. May be I'll try to stain the cells will CFSE and culture with IL-2 for a few days.