Connection between Matlab and visual studio?

i am working in transfer of images between two computer . I don't know how to make connection between Matlab and visual studio . i used a visual basic for programming my controller. Can anyone...

31 December 2020 6,708 4 View

What is the reason of suddenly increasing the concentration while evaluating the photocatalytic performance by RhB dye degradation?

I am doing a photocatalytic performance evaluation by RhB dye degradation. I am facing a problem which is: After achieving the absorption -desorption equilibrium, I checked the concentration and...

04 December 2020 3,922 20 View

NOVA 2.0 - Advanced Electrochemistry Software?

How can you help me to get a program NOVA 2.0 - Advanced Electrochemistry Software?

26 November 2020 3,873 4 View

What are the investigations that should be done to the patient suspected to be infected with corona virus( COVID -19) other than PCR?

What are the investigations that should be done to the patient suspected to be infected with coronavirus( COVID -19) other than PCR?

02 July 2020 8,746 7 View

How do you think that assessment for practical parts of any subject could be achieved by online means,and how far it would measure the student skill?

Due to covid-19 breakdown, most of education systems converted to whole online learning, and as the final semester for the current year is on the end, assessments must be done to measure if the...

29 May 2020 2,521 7 View

SDN Router Selection how to implement using python ?

algortihm 1 basic link selection input: txm matrix M, integer k output: MP =(M1 M2) where M1 is txk 1: compute the SVD of M: M= Udelta V T; 2: Apply QR with column pivoting to first k column...

16 May 2020 8,346 7 View

SDN router placement using python algortihm ? and Basic Link Selection of SDN?

I need SDN router placement algorithm with python code, i need python code to generate SDN router graph and charts but i did not want to use NS3 and Mininet . I wants to use only window 10...

16 May 2020 4,387 3 View

What materials are usually chosen for efficient gas sensing(at least 3 different metal oxides as well as the precursor materials)?

I have just started to gain basic information about gas sensing materials. So here, I want to know what materials are in touch nowadays for efficient gas sensing, and what are the corresponding...

11 May 2020 5,277 1 View

What is the number of feathers colors in quail?

What is the number of feathers colors in quail?

02 March 2020 4,642 2 View

Can I use predictive data from neural network in isotherm model ?

can I use predictive data from neural network in isotherm model I need more points can I get this points from ANN and use in isotherm model

20 September 2019 4,814 2 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View

Is there a way to extract total RNA and protein from cell culture at the same time, without denaturing protein?

Hi all, Is there a way to extract RNA and protein from the same cell culture sample? Because we need to use the protein for gelatin zymography at later time, we are looking for a method that can...

22 February 2021 5,341 5 View