Aasim Majeed

10 Questions 9 Answers 0 Followers

Questions related from Aasim Majeed

Require suggestion on calculating Mutation Response Index for determining mutational role on bias?

I am working on codon usage bias on my species. While other indices and analysis have been carried out successfully, I have a confusion regarding the MRI calculation. MRI = SCS - CSCS For...

20 November 2019 3,257 1 View

Query about Mratio test and inferring genetic bottleneck in Populations?

I conducted Mratio analysis on my data set using M_P_Val.exe . It gave me following result; Average M = 0.110098 Please wait... 0% of the time you expect a smaller ratio at equilibrium. Using...

21 May 2019 1,706 3 View

What is the difference between M and Mc while using Mratio test for finding genetic bottle neck?

The value of M defines if a population has undergone recent reduction. There is another term called as critical M or Mc apart from M. What does it mean and how it is related to M. Thanks

04 May 2019 648 0 View

Is there any pipeline for detecting, quantifying and comparing alternative splicing from RNA seq data of plants?

I need to detect, quantify, identify differential splicing, and compare the alternative splicing across different RNA seq datasets of plants. I am the beginer in this field. Please suggest the...

05 February 2016 1,704 2 View

Can anyone tell me about the bet tools for alternative splicing detection and quantification?

From the transcriptomic data of a plant generated under different physiological conditions,I need to analyze the alternative splicing including its quantification. What insilico strategy would...

04 February 2016 10,094 2 View

How can I perform transient expression using Agrobacterium?

In the literature people have used agrobacterium mediated transformation of protoplasts for genome editing via CRISPR-Cas9 technology to transiently express the sgRNA-Cas9 cassette. For stble...

12 October 2015 2,611 4 View

I have gRNAs fo specific barley genes. Which plasmid will work best for me?

I want to knock down few genes in barley, I have designed gRNAs for them. Now there is the issue of plasmid selection that could be suitable for Agrobacterium mediated gene transfer. From Addgene...

30 September 2015 725 1 View

Is there any host gene in wheat or barley that confers susceptibility to Bipolaris sorokiniana?

Bipolaris sorokiniana is a biotrophic fungi causing spot blotch in wheat and barley. Do any host gene help this fungus during infection.

09 June 2015 3,146 2 View

Are there any known genes in crops especially in wheat and rice that help pathogen during infection?

I need to know about any host genes that promote pathogen infection.

02 June 2015 7,403 3 View

Do leaves influence grain filling in cereals like stem and spike leaves?

I want to perform transcriptomic and metabolomic analysis of components of grain filling. Apart from photosynthates, do metabolites mobilize from leaves into seeds during grain filing and leaf...

08 May 2015 6,701 1 View