I want to induce MRP4 in different cell lines by using some specific inducing agents. Does anybody know about any specific inducing agents for MRP4?
To my mind, you should consult the paper: ''Induction of hepatic transporters multidrug resistance-associated proteins (Mrp) 3 and 4 by clofibrate is regulated by peroxisome proliferator-activated receptor alpha'' (PMID: 20405259)
I am trying to perform HE staining of small intestine. I used OCT solution to make sections. After HE staining when I look my slides under microscope, I do not get intact villi. In much of the...
01 February 2020 5,960 3 View
We are currently working on a cytosolic protein; however, we think that this protein also has a secreted form and can be detected in the bloodstream. As there is no ELISA kit available for this...
01 February 2020 468 2 View
I want to study whether two proteins: Myc-tagged protein A and Flag-tagged protein B, interacts with each other or not. For that I co-expressed these proteins in HEK293T cells, and later (after 48...
01 February 2019 1,927 4 View
I want to determine whether protein A and protein B interacts with each other by co-immunoprecipitation analysis. For that I prepared Myc-tagged protein A and Flag-tagged protein B plasmid DNAs. I...
01 February 2019 994 4 View
I am just wondering whether different labs prepare immortalized bone marrow derived macrophages (BMDM) in their labs. I have experience of preparing primary BMDM from mice femur, but have no idea...
31 December 2018 8,956 2 View
I have been working on two proteins. And I think that these two proteins interact with each other. To prove that, I analyzed there association by endogenous IP, but unfortunately I could not get...
11 December 2018 9,770 4 View
Articles published in journals contains both reference and citation, while books contain only reference. Does anybody have idea about it?
09 October 2018 971 0 View
I just started to do EMSA to check interaction of a protein and DNA. When I checked the binding buffer used for EMSA, I found that EDTA is also used. I know that EDTA is a chelating agent, and...
04 May 2018 2,719 1 View
I have been recently trying to isolate primary microglia from mice pups brain (P0-P1). To prepare mixed microglia culture, I used 3 brains for a 75 cm2 flask. After around 10-12 days, astrocyte...
03 April 2018 3,801 12 View
Currently, I am doing knockdown of certain genes by shRNA technique. We use lenti-virus for this process. The problem is that we are getting low titre. The experimental procedure is: Day 1: Seed 7...
04 May 2017 2,452 6 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View