I want to induce MRP4 in different cell lines by using some specific inducing agents. Does anybody know about any specific inducing agents for MRP4?
To my mind, you should consult the paper: ''Induction of hepatic transporters multidrug resistance-associated proteins (Mrp) 3 and 4 by clofibrate is regulated by peroxisome proliferator-activated receptor alpha'' (PMID: 20405259)
Does anybody know how many endothelial cells are present in the heart of an adult mouse?
23 July 2023 8,201 1 View
Does anyone know the diameter (size) of mouse heart endothelial cell?
08 July 2023 1,480 1 View
I want to know whether LPS+ATP combination induces inflammasome activation in HUVEC or endothelial cells. Thanks
08 March 2023 5,173 1 View
I'm using a cycloheximide chase assay to look into the stability of a protein. Despite giving the cells cycloheximide (100 uM) over a number of time periods (0, 12h, 24h, and 36h), I was unable to...
19 January 2023 234 0 View
Greetings everyone I am trying to etch some additively manufactured CuCrZr alloys but i am not able to get the melt pool can anyone give me some suggestions I have tried with FeCl3 HCL...
16 January 2023 4,409 4 View
I need to understand the processing route of non-equiatomic high entropy braze foils
18 September 2022 9,198 0 View
I want to make a stacked bar graph with showing each data value as dots. I tried with graph pad prism, version 6, but could not succeed. Does anyone have tried before making such graphs?
01 March 2021 9,899 1 View
I performed HE staining of intestinal section of wild type (WT) and Knockout mice (KO). I found that the staining intensity is reduced in case of KO mice. There was no difference in villi length...
20 July 2020 1,442 0 View
I am working on a study to understand tourists poor behaviour towards beach cleanliness. For that I am collecting quantitative data. As part of the research, I am also willing to understand the...
15 July 2020 7,494 15 View
I am trying to perform HE staining of small intestine. I used OCT solution to make sections. After HE staining when I look my slides under microscope, I do not get intact villi. In much of the...
15 February 2020 2,456 4 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View