Dear all,
I would like to ask if anyone know how to detect total PDE (phosphodiesterase) activity in mouse liver
I know that we can measure PDE individually by western blot and qPCR which are very laborious and time-consuming.
Thanks
Hi community, I am doing my phd in Nanoelectronics, I just started it and I am doing Bibliography part. With regard to this I was learning about the 12 term error term model for 2 port network...
21 December 2023 2,510 3 View
I am doing research on diagnosis for dark skin people. However it's really difficult to acquire the dark skin disorders’ images, please tell me the ideas to get the dark skin disorders’ images...
27 March 2022 1,884 0 View
I want to use MOCVD to grow ohmic contact electrode layer of n-type GaN, but all the literatures I have read are grown by sputtering or evaporation method. Can the electrode be prepared by CVD...
31 August 2020 4,622 3 View
Dear all, I would like to ask the method to extract cAMP from mouse liver. I found some papers homogenizing the tissue in HCl. Is this the most appropriate method? Thanks
12 March 2020 632 1 View
Hi all, I am producing AAV vector-containing shRNA to knockdown my target protein. Three pDNAs (transfer plasmid, pHelper, RepCap) are transfected into HEK293A (80% confluency). I can get a very...
27 November 2019 2,339 6 View
Hello all, I want to knock down the gene using siRNA. As siRNA of my targeted gene is commercially available in life technologies. Although there are many candidates available, there possess...
29 January 2018 7,622 4 View
Hi all, I am writing this question to inquire about the isolation of mouse primary hepatocytes. As I am a completely beginner, after isolating the cells, it turn out that the viability is 50%...
08 November 2017 6,644 7 View
Hello, I would like to inquire if anybody has the protocol for nuclear extraction of mouse liver. Thanks
24 October 2017 8,778 3 View
Hello all, I hope all of you are doing fine. As we know well that Glucagon and Glucocorticoid activate hepatic gluconeogenesis via cAMP. I found a paper using cAMP/dex to treat to cultured cell...
13 August 2017 9,201 0 View
Hello everyone, I am conducting the qPCR to check the gene expression. In spite of high reaction efficiency of the housekeeping gene, my gene of interest amplifies very low as the ct value is...
29 June 2017 9,817 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...
05 August 2024 3,246 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...
30 July 2024 7,077 1 View
Dear researchers. I tried using the IHC PROFILER in image j to quantify nuclear DAB staining. I followed the instructions in the original article by "Varghese F, Bukhari AB, Malhotra R, De A...
29 July 2024 2,229 0 View
Dear All, here is my question: if I have a 15-scans cyclic voltammetry performed with PalmSens, how can I obtain the charge passed during the process? Hopefully avoiding integrations,...
29 July 2024 3,733 5 View
My question pertaining to the DAB staining in cytoplasm of human oral squamous cell carcinoma tissue. When quantifying the epithelial cancer cells do we have to crop remove the stromal tissue?...
29 July 2024 2,682 6 View
I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...
23 July 2024 8,492 3 View
I am currently conducting a mendelian randomization study, and I was attempting to use PhenoScanner to look for potential confounders associated with the selected SNPs (any SNPs significantly...
23 July 2024 5,703 0 View