CUDA Out Of Memory (OOM) error while using GPU?

I'm training a neural network (E3D-LSTM) for spatio temporal video prediction. It's quite a big network that has millions of parameters. The model is training perfectly when using my 12 CPU cores,...

25 December 2020 1,229 3 View

Is there any integrated genome browser available to see chloroplast gene expressions?

Dear colleagues, Good Day! I am looking for any public database for visualizing RNA-seq data of chloroplast genes. For ex. I want to see the expression of Polycistronic mRNA abundancies of genes...

20 December 2020 7,474 3 View

Need airfoil coordinates?

Hi all, I hope you all are doing well. I am working on the simulation of airfoils with boundary layer suction. I found a reference to validate my CFD results, as shown below. "Stanewsky E,...

06 September 2020 9,013 3 View

Porous media coefficients and working fluid?

Hi all, I hope you all are doing well! I am wondering if the porous media coefficients will change if the working fluid changes. I have a tubular, U-shaped heat exchanger geometry where the...

01 July 2020 246 3 View

What is the adiabatic heat transfer coefficient, and how to estimate it using CFD?

Hi everyone, I hope you all are doing well. Can anybody in the forum explain what adiabatic wall temperature and heat transfer coefficients are? More importantly, how to estimate it using CFD...

25 March 2020 4,644 11 View

Why it needs long time exposure to develop/get to see band images in western blot?

Hello Everyone, I'm checking expression for 4-HNE (abcam) (1:2000) by western blotting in mice skin tissue against UV stress. I'm facing struggle to get good visible bands, as I get band view only...

18 March 2020 5,800 3 View

Can we use DNA as template to do real-time PCR to check expression of gene?

Hello Everyone, I wanted to run RT-PCR using onestep real-time PCR systems and SYBR green (ThermoFischer) to check the gene expression in bacteria. I wanted to directly use 50 ng of DNA samples...

10 March 2020 1,965 3 View

Dependence of magnetic moment on the number of unpaired electrons?

I ran VASP relax calculations for Fe in the interstitial position of MgO for different charge states of Fe from 0 to +3. The magnetic moment seems to increase from 2BM to 5BM in steps of 1 as each...

26 August 2019 1,863 2 View

Modelling thermosyphon in Star-CCM+?

Hi all, I am trying to model a thermosyphon (which is a heat transfer device based on evaporation and condensation processes) using Star-CCM+. The details were taken from this...

06 June 2018 3,624 3 View

Tetra meshing for flow past an aircraft (DLR F6) wing in ICEM CFD?

Hi everyone, I am trying to mesh the wing of an aircraft (DLR F6) using ICEM CFD (tetra) in accordance with the guidelines prescribed by Drag Prediction Workshop. I am quite new to ICEM CFD and...

21 January 2018 8,950 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

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17 February 2021 4,217 3 View

Which detection method is better for an ELISA-based assay: colorimetric or fluorometric?

Hello, I am planning to perform an ELISA-based assay to quantify the percentage of genomic methylation (5-methylcytosine) in human embryonic stem cells. I've been searching for some kits and I...

16 February 2021 8,389 3 View