Hariharan Balasubramaniam

4 Questions 7 Answers 0 Followers

Questions related from Hariharan Balasubramaniam

Why there are peaks and gaps on EXON regions on RNA seq- data?

Hello everyone! I have interesting question asked by my professor and I could not find relevant answer anywhere. Why are we seeing up and down pattern on transcript abundance? Example RNA seq data...

20 January 2023 9,259 4 View

Is there any method or kit available to study DNA methylation in the context of CHH?

Hello everyone! I am looking for a quick and dirty method to study DNA methylation in the context of CHH (H is A T or C) methylation similar to CHOP PCR? Right now i am in a preliminary stage...

17 December 2021 1,888 0 View

Is there any integrated genome browser available to see chloroplast gene expressions?

Dear colleagues, Good Day! I am looking for any public database for visualizing RNA-seq data of chloroplast genes. For ex. I want to see the expression of Polycistronic mRNA abundancies of genes...

21 December 2020 7,656 3 View

Why it is necessary to clone a plant gene in an entry vector before shuttling into an expression vector?

Hello Everyone! I am new to plant molecular biology and i have a doubt. Is it always necessary to clone a plant gene in an entry vector (such as pJET, pGEM®-T Easy vector) before cloning into a...

01 January 1970 5,115 4 View