How can I perform RNA isolation from MRSA without using a kit?

I am trying to isolate RNA from Trizol and triton x but results are not coming, so help me out. Thanks

07 August 2014 6,589 5 View

How do I know if I have a problem with my primers or PCR?

I have taken HLA B27 specific primers from literature and tried to amplify them on DNA of an arthritis patient and there was no amplification. There was no amplification, i want to know that...

03 April 2014 9,379 12 View

Do anybody have an HPLC method for the detection of Cortisol in hairs?

Please share if you have.

03 April 2014 2,133 3 View

Is it necessary that if we have HLA B27 specific primer, it will amplify in a normal individual manner?

If yes why , please describe briefly.

03 April 2014 4,349 0 View

Can anyone help with my hemolysis problem?

I did hemolysis with my peptide at different concentration and it was observed that at high concentration the hemolysis was suppressed as compared to low concentration. I am not getting any...

03 April 2014 7,511 0 View

Which antigen i.e O, H or Vi should be taken to identify typhoid and why?

I am not able to find out the antigen specific for typhoid detection.

03 April 2014 341 1 View

How can we identify HLA present on the klebsiella residing in the human body?

Literature shows that klebsiella is also playing a part in arthritis, so can we identify klebsiella HLA by amplyfying with the primers use to amplify HLA on human PBMC.

03 April 2014 5,631 16 View

Does anyone know where I can get inhibitors of HLA b27, which are soluble in water?

Thanks.

02 March 2014 7,717 2 View

Can someone suggest a cELISA protocol?

For expression studies.

02 March 2014 6,133 5 View

Can anyone help with PBMC from whole blood by Ficoll method, I dissolved the pellets in PBS?

Viability was 57%, but how do I know that the cells shown are viable PBMC cells and as these are stored in PBS can I directly add a lysis buffer to extract DNA from these PBMC stored in PBS?

02 March 2014 5,520 4 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

No title

02 March 2021 3,060 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View