Saurabh Pandit

15 Questions 23 Answers 0 Followers

Questions related from Saurabh Pandit

How can I perform RNA isolation from MRSA without using a kit?

I am trying to isolate RNA from Trizol and triton x but results are not coming, so help me out. Thanks

08 August 2014 6,655 5 View

Can anyone help with my hemolysis problem?

I did hemolysis with my peptide at different concentration and it was observed that at high concentration the hemolysis was suppressed as compared to low concentration. I am not getting any...

04 April 2014 7,607 0 View

How do I know if I have a problem with my primers or PCR?

I have taken HLA B27 specific primers from literature and tried to amplify them on DNA of an arthritis patient and there was no amplification. There was no amplification, i want to know that...

04 April 2014 9,441 12 View

Is it necessary that if we have HLA B27 specific primer, it will amplify in a normal individual manner?

If yes why , please describe briefly.

04 April 2014 4,417 0 View

How can we identify HLA present on the klebsiella residing in the human body?

Literature shows that klebsiella is also playing a part in arthritis, so can we identify klebsiella HLA by amplyfying with the primers use to amplify HLA on human PBMC.

04 April 2014 5,696 16 View

Which antigen i.e O, H or Vi should be taken to identify typhoid and why?

I am not able to find out the antigen specific for typhoid detection.

04 April 2014 405 1 View

Do anybody have an HPLC method for the detection of Cortisol in hairs?

Please share if you have.

04 April 2014 2,196 3 View

After having pellet from the whole blood by ficoll paque method, how to proceed for RNA isolation?

I want to isolate RNA by trizol method. I have got pellet from whole blood by ficoll paque method. Now, how shall I proceed with those pelles?

03 March 2014 9,247 2 View

Can someone suggest a cELISA protocol?

For expression studies.

03 March 2014 6,210 5 View

Can anyone help with PBMC from whole blood by Ficoll method, I dissolved the pellets in PBS?

Viability was 57%, but how do I know that the cells shown are viable PBMC cells and as these are stored in PBS can I directly add a lysis buffer to extract DNA from these PBMC stored in PBS?

03 March 2014 5,585 4 View

Hello, what should the concentration of NEM (N-ethylmaleimide) be to block thiol groups on PBMCs?

Thiol blockers.

03 March 2014 2,588 1 View

I have to block thiol groups present on the surface of a HLA antigen, can anyone recommend a non-toxic blocker soluble in water?

Please name the most efficient blockers.

03 March 2014 8,017 2 View

Is there any method for identifying HLA B27 antigens in peripheral mononuclear cells?

The method should identify the HLA antigen before DNA isolation.

03 March 2014 4,772 4 View

Does anyone know where I can get inhibitors of HLA b27, which are soluble in water?

Thanks.

03 March 2014 7,782 2 View

Will I be able to produce different antibodies against mixture of antigens i.e one culture having different enzyme variants?

02 February 2014 1,037 0 View