15 Questions 23 Answers 0 Followers
Questions related from Saurabh Pandit
I am trying to isolate RNA from Trizol and triton x but results are not coming, so help me out. Thanks
08 August 2014 6,653 5 View
I did hemolysis with my peptide at different concentration and it was observed that at high concentration the hemolysis was suppressed as compared to low concentration. I am not getting any...
04 April 2014 7,601 0 View
I have taken HLA B27 specific primers from literature and tried to amplify them on DNA of an arthritis patient and there was no amplification. There was no amplification, i want to know that...
04 April 2014 9,438 12 View
If yes why , please describe briefly.
04 April 2014 4,413 0 View
Literature shows that klebsiella is also playing a part in arthritis, so can we identify klebsiella HLA by amplyfying with the primers use to amplify HLA on human PBMC.
04 April 2014 5,693 16 View
I am not able to find out the antigen specific for typhoid detection.
04 April 2014 401 1 View
Please share if you have.
04 April 2014 2,191 3 View
I want to isolate RNA by trizol method. I have got pellet from whole blood by ficoll paque method. Now, how shall I proceed with those pelles?
03 March 2014 9,243 2 View
For expression studies.
03 March 2014 6,204 5 View
Viability was 57%, but how do I know that the cells shown are viable PBMC cells and as these are stored in PBS can I directly add a lysis buffer to extract DNA from these PBMC stored in PBS?
03 March 2014 5,583 4 View
Thiol blockers.
03 March 2014 2,584 1 View
Please name the most efficient blockers.
03 March 2014 8,012 2 View
The method should identify the HLA antigen before DNA isolation.
03 March 2014 4,768 4 View
Thanks.
03 March 2014 7,778 2 View
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02 February 2014 1,032 0 View