Viability was 57%, but how do I know that the cells shown are viable PBMC cells and as these are stored in PBS can I directly add a lysis buffer to extract DNA from these PBMC stored in PBS?
My question would be how did u assess the viability?
if you did it microscopically you can actually observe and verify the morphology thus, answering your question.
As for lysis, a short low speed centrifugation will pellet the PBMC after which you can remove the PBS supernatant and add your lysis buffer.
Or maybe I did not understand your question.
How was viability determined? If it was via trypan blue exclusion method you can be able to enumerate viability and even the numbers. But if you keep the PBMCs in PBS for long without any media they will eventually die.
For DNA extraction follow Jay's suggestion it will pellet most of the cells. Point to note check the type of centrifuge your are using whether fixed head or swing out.
I hope i have given some useful info.
Thanks for the suggestions. I assess the viability by trypan blue method.
viability must increase is it? You must take off only interface blurred level by disposible plastic pipet and wash three times with PBS.
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