Is it necessary that if we have HLA B27 specific primer, it will amplify in a normal individual manner?

Saurabh Pandit @Saurabh_Pandit

04 April 2014 0 4K Report

If yes why , please describe briefly.

Article HLA-B27 typing: Evaluation of an allele-specific PCR melting...

Badges
Science topic

More Saurabh Pandit's questions See All

How can I perform RNA isolation from MRSA without using a kit?

I am trying to isolate RNA from Trizol and triton x but results are not coming, so help me out. Thanks

07 August 2014 6,681 5 View

Do anybody have an HPLC method for the detection of Cortisol in hairs?

Please share if you have.

03 April 2014 2,224 3 View

How do I know if I have a problem with my primers or PCR?

I have taken HLA B27 specific primers from literature and tried to amplify them on DNA of an arthritis patient and there was no amplification. There was no amplification, i want to know that...

03 April 2014 9,472 12 View

Which antigen i.e O, H or Vi should be taken to identify typhoid and why?

I am not able to find out the antigen specific for typhoid detection.

03 April 2014 427 1 View

Can anyone help with my hemolysis problem?

I did hemolysis with my peptide at different concentration and it was observed that at high concentration the hemolysis was suppressed as compared to low concentration. I am not getting any...

03 April 2014 7,650 0 View

How can we identify HLA present on the klebsiella residing in the human body?

Literature shows that klebsiella is also playing a part in arthritis, so can we identify klebsiella HLA by amplyfying with the primers use to amplify HLA on human PBMC.

03 April 2014 5,727 16 View

Does anyone know where I can get inhibitors of HLA b27, which are soluble in water?

Thanks.

02 March 2014 7,811 2 View

Can someone suggest a cELISA protocol?

For expression studies.

02 March 2014 6,238 5 View

Can anyone help with PBMC from whole blood by Ficoll method, I dissolved the pellets in PBS?

Viability was 57%, but how do I know that the cells shown are viable PBMC cells and as these are stored in PBS can I directly add a lysis buffer to extract DNA from these PBMC stored in PBS?

02 March 2014 5,614 4 View

I have to block thiol groups present on the surface of a HLA antigen, can anyone recommend a non-toxic blocker soluble in water?

Please name the most efficient blockers.

02 March 2014 8,045 2 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View