If yes why , please describe briefly.
Article HLA-B27 typing: Evaluation of an allele-specific PCR melting...
I am trying to isolate RNA from Trizol and triton x but results are not coming, so help me out. Thanks
07 August 2014 6,589 5 View
I have taken HLA B27 specific primers from literature and tried to amplify them on DNA of an arthritis patient and there was no amplification. There was no amplification, i want to know that...
03 April 2014 9,379 12 View
Please share if you have.
03 April 2014 2,133 3 View
I did hemolysis with my peptide at different concentration and it was observed that at high concentration the hemolysis was suppressed as compared to low concentration. I am not getting any...
03 April 2014 7,511 0 View
I am not able to find out the antigen specific for typhoid detection.
03 April 2014 341 1 View
Literature shows that klebsiella is also playing a part in arthritis, so can we identify klebsiella HLA by amplyfying with the primers use to amplify HLA on human PBMC.
03 April 2014 5,631 16 View
Thanks.
02 March 2014 7,717 2 View
For expression studies.
02 March 2014 6,133 5 View
Viability was 57%, but how do I know that the cells shown are viable PBMC cells and as these are stored in PBS can I directly add a lysis buffer to extract DNA from these PBMC stored in PBS?
02 March 2014 5,520 4 View
Please name the most efficient blockers.
02 March 2014 7,952 2 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why
02 March 2021 1,388 3 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?
28 February 2021 8,095 1 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...
26 February 2021 5,029 2 View
Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
25 February 2021 6,969 3 View
Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...
24 February 2021 1,243 1 View