I am looking for a protocol for induction of acyclovir resistance in HSV1 in epithelial cell line. Please share with me if you have any.
Hi, please see the following article
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC145299/
Data Acquisition detected more than 65000 metabolites with area maximum, RT-value and peak-rating. What is the significance of Peak Rating?
07 July 2024 6,643 2 View
I want to calculate the CBM and VBM from the band gap of NaLa(MoO4)2. But is unable find out the absolute electronegativity of NaLa(MoO4)2. Please help me with proper references How can I...
23 November 2023 9,701 1 View
Confocal imaging of cucumber leaf mesophyll cells by ROS I am using a 25 uM concentration with 30 minutes of incubation in the dark.
05 November 2023 8,032 1 View
Dear all Post docking I am not able to see any hydrogen bond interaction in my complex in ligplot. While when I visualised the same complex in DSV studio able to see two three conventional...
07 October 2023 5,145 2 View
Hello everyone I am facing a issue with complex visualisation in ligplot and pymol.. When I tried to open the docked PL complex in the aforementioned tools only ligands are visible and no...
03 September 2023 1,610 4 View
I need COV434 cells for toxicity study in human ovarian granulosa cells.
04 August 2023 5,182 0 View
I've gone through a large number of literature but couldn't find relates rather saying crosslink density would affect it. Would you mind helping me with your knowledge to resolve this or help me...
25 July 2023 4,036 0 View
What experimental conditions or methods can be employed to induce the formation of multiple polymorphs in a cocrystal that previously exhibited various polymorphs with a specific solvent under...
04 July 2023 7,770 1 View
Hello, I am engaged in a research related to leishmaniasis and expect to develop a model based on the qualitative and quantitative findings of my study. But the challenge is the lack of literature...
29 June 2023 7,037 4 View
Both components show maximum wavelength at 280 nm. How to calculate the concentration of both the components in that solution
14 June 2023 8,811 16 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View