What should be Optimum Review duration for a research/review article for any [unpaid/freely publishing]journal till acceptance?

Dear Research Gate Members, We all have experience regarding publications of research/review articles in various journals. Some of the journals review the articles in 4-6 weeks and some takes...

01 October 2020 7,201 1 View

How to write an impressive review paper in the field of Industrial effluent treatments?

I am currently working on collecting literature based on the available treatment technologies for industrial effluents. There are vast variety of methods physical, biological and Advanced...

23 September 2020 2,314 4 View

How to check if a time series data is stationary using MATLAB ?

Flood frequency analysis is mainly based on the assumption of stationarity of the data; however, in recent years, this assumption has been open to doubt. So before carrying out the FFA study, what...

07 September 2020 2,586 3 View

Is log i vs log v (scan rate) an appropriate parameter for defining the capacitive and battery behaviour.?

According to the power law i=av^b, for a material (reduced graphene oxide) to be capacitive the value of b=1 whereas b=0.5 for battery like behaviour. However, the CV shows a quasi rectangular...

26 February 2020 4,592 4 View

How to calculate LOD & LOQ in the validation of analytical method using HPLC?

I have injected 5 samples of lowest possible concentrations. I have taken the std deviation of area and slope of the equation obtained for the regression line of calibration to calculate LOD &...

19 January 2020 4,730 7 View

Can I use the haploview to test linkage disequilbrium for around 10 SNPs?

I would like to perform linkage disequilibrium test for a number of SNPs. I am absolutely new to this field and would like to know what input file should be taken for my SNPs. I also would like to...

11 December 2019 8,416 2 View

Is the process of administration of dopaminergic neuron after its formation from skin fibroblasts to Hippocampus of Brain possible?

Current researches to cure Parkinson's disease deal with the formation of the dopaminergic neurons from the Skin fibroblasts but even after the formation of neurons, is their administration to the...

25 August 2019 3,585 2 View

Can pre-trained networks of ML/DL be used for research publications?

Hey, Imagine a scenario, I am required to train machine learning model on ABC data-set. There are few pre-trained models trained on same ABC data-set. Can I use these pre-trained models in my...

26 January 2019 2,057 8 View

A colored drug interference with TSA causing problem to determine binding pattern of compound?

HI, I am screening compounds against my synthetase enzyme with thermal shift assay. I have encountered a drug which is showing a shift of around 20degrees from the Tm of my protein. I am using 2X...

22 January 2019 4,457 1 View

Marker for neuroinflammation or microglial activation in peripheral blood?

Can peripheral blood be used to check neuroinflammation or microglial activation? or Do we have any markers in the peripheral blood which can detected either by ELISA or flow cytometry and give us...

17 August 2018 6,015 7 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View