How to check if a time series data is stationary using MATLAB ?

Flood frequency analysis is mainly based on the assumption of stationarity of the data; however, in recent years, this assumption has been open to doubt. So before carrying out the FFA study, what...

07 September 2020 2,586 3 View

Is there any literature on induction of acyclovir resistant HSV1 in cell lines?

I am looking for a protocol for induction of acyclovir resistance in HSV1 in epithelial cell line. Please share with me if you have any.

02 June 2018 1,190 1 View

Can the oncogenic or tumor suppressor function change with cellular context? Would this have implications for cancer biology/therapy ?

For example if an oncogene is within a cell that lacks components of the downstream signaling pathway, then it will not function as an oncogene anymore. Could this be the reason that oncogenes...

01 January 2018 9,079 12 View

Is there a web interface for 2D classification and building 3D structure from Cryo-EM images?

Is there a web interface for 2D classification and building 3D structure from Cryo-EM images?

08 November 2017 2,567 2 View

Is biology of primary tumor opposite to/different from epithelial-mesenchymal transition biology ?

Note: EMT (epithelial mesenchymal transition) and MET (mesenchymal epithelial transition) are opposing processes .Therefore should have theoretically opposite biologies.

21 August 2017 7,161 3 View

We want to measure salinity of water column at different depths, is it possible to do using an automatic analyzer?

Measuring salinity at different depths at the same time. One way is to install salinity loggers at different depths. Wondering if there was another method.

27 October 2016 3,544 3 View

Why in SEM (Scanning Electron Microscopy) with increase in temperature Grain size increases ?

04 July 2016 7,683 10 View

I would like suggestions that which sample I should collect from the cancer patients, blood, sputum or urine in order to cover essential cytokines.

I am working on comparative study of cytokines secreted during cancer growth in cancer patients. I will extract the samples and would perform HPLC and OD for estimation of cytokines.

15 March 2016 4,171 3 View

Dna extraction

05 March 2016 1,676 4 View

How can I troubleshoot P1 phage transduction?

I am trying to carry out gene deletion using P1 phage transduction in Ecoli W3110 Strain. Here I have successfully generated lysate from the donor strain with pfu 1.0E+9/ml via plate method. I am...

23 February 2015 2,846 7 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

How to model Supercritical water gasification (SCWG) process in Homer software?

Hi everyone How to model Supercritical water gasification (SCWG) process in Homer software?

25 February 2021 4,075 1 View