I have purified a protein using a protocol provided in a reasearch paper but this protein unable to bind to RNA (emsa) used as a positive control.Why?

The protein purified by them is showing binding to the RNA. I have used exactly same lysis, washing and elution buffer. I have got purified very good purified protein.

03 April 2018 9,634 10 View

Any online software or database available that provides information about loss of heterozygosity of a gene in a cell line?

If one is interested to know whether a gene resides in loss of heterozygosity region of a cell line, what are the ways he/she can resolve it? Is there any database or online bioinformatic tool is...

10 November 2016 1,271 1 View

How to use the NCBI GEO analysis/data-sets to find out expression profile of a particular gene?

In research articles people often show meta analysis of a gene using NCBI GEO data sets or analysis, i want to know the procedure by which i can derive the information for a particular gene?

01 February 2015 2,395 3 View

What is "Application Note" in a journal, does it have same value as research article publication?

I want to understand the importance of :Application note" section of a journal, Is it same as publishing a research article and have the same value (in terms of Impact factor of that journal) as...

31 December 2014 6,059 2 View

Ethical and technical issue

07 August 2014 7,114 1 View

Can we consider HEK 293T as a normal cell line?

If one is performing some experiments with cancerous cell lines, will it be a correct strategy to use HEK 293T as a normal cell line as a control?

02 March 2014 1,677 13 View

Concentration of L-Lysine and L-Arginine in normal RPMI and DMEM?

Can anybody inform me the concentration of L-Lysine and L-Arginine in normal RPMI and DMEM media?

01 February 2014 10,015 7 View

How to calculate real-time PCR data?

I am confused about how to calculate the fold change of transcript level by real time PCR, suppose in two conditions: control : GAPDH - 25 gene X- 18 Test : GAPDH - 23 gene X - 20 What will be...

31 December 2013 1,167 14 View

What are the ways a person can test the drug resistance for a particular cell line for a particular drug?

What are the experimental methods one can perform to find out whether a cell line is really resistance to a certain drug or not?

10 November 2013 1,163 3 View

How to remove the stains of developer from cloths?

Its a silly question.. one of my colleague asked me this question, but I couldn't answer so I am asking it here

07 August 2013 1,863 1 View

Greetings to all, has any body published a scientific paper in EuroAsian Journal of BioSciences?

Greetings to all, has any body published a scientific paper in EuroAsian Journal of BioSciences and if so could you please tell me if there was any problems regarding communications with the...

05 October 2020 1,713 2 View

Do you agree with the just issued Great Barrington Declaration regarding the response to COVID-19?

The short and to-the-point text is here and I for one gladly co-signed this declaration, just as I also co-signed the balanced approach statement issued in July 2020 by 23 public health experts...

04 October 2020 8,795 11 View

The meaning of protein phosphorylation to bioscience?

Many researchers have mentioned in their researches that protein phosphorylation take a decisive position to regulate various physiological activity, from prokaryote to eukaryote. So did...

23 July 2020 7,135 3 View

Is there any cause of pellet from the culturing media?

11 June 2020 3,585 3 View

How do you reduce the static forces in canon ball packing of fruits for transportation?

Examples of how to increase the contact surface area of the bottom area? Thank you.

25 March 2020 9,187 4 View

How molecular genetics could be useful for farmers?

Molecular genetics is the branch of genetics that investigates the chemical and physical nature of genes and the mechanisms by which genes control development, growth and physiology. It is the...

03 January 2020 3,565 4 View

How molecular genetics could be useful for farmers?

Molecular genetics is the branch of genetics that investigates the chemical and physical nature of genes and the mechanisms by which genes control development, growth and physiology. It is the...

31 December 2019 3,792 4 View

Has anyone successfully stained (intracellular staining) for T-bet with the Cytofix/Cytoperm Kit from BD Biosciences??

I isolate PBMCs from human blood and try to perform intracellular staining for T-bet (i'm interested in CD19+ cells, but for now i use CD3+ as a control). I do know there are kits that are...

22 September 2019 2,889 3 View

Has anyone successfully stained (intracellular staining) for T-bet with the Cytofix/Cytoperm Kit from BD Biosciences??

I isolate PBMCs from human blood and try to perform intracellular staining for T-bet (i'm interested in CD19+ cells, but for now i use CD3+ as a control). I do know there are kits that are...

08 September 2019 5,009 3 View

So what you think that either you will choose to develop a desktop application, mobile application or the application that can run on both platforms ?

As internet usage increase web application development become a challenge, because it is mostly customer requirement that web application must be usable on both desktop computer and...

07 August 2019 1,193 3 View