Is there any limitations of performing a Western blot analysis of transiently transfected cells? can you trust these blots?

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Chandra Somasundaram

... protein expression from transiently transfected cells

No.

yes.

qRT-PCR, immunofluorescence

Majid Jabir

You could confirm this test by IF, and RT-PCR techniques.

Hamadi Madhi

One technique is not enough. You should confirm your western results by adding other experiments such as Q-PCR, immunocytochemistry and siRNA.