Sarah Johnson

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Questions related from Sarah Johnson

Unexpected band seen with anti-p53 antibody (DO-1)?

I transfected cells with three different plasmids expressing p53 gene. I also had non-transfected cells. I performed western blot of the whole-cell lysates and probed for p53 using DO-1 antibody....

01 January 2020 7,710 3 View

Is there any limitations of performing a Western blot analysis of transiently transfected cells? can you trust these blots?

Is there any limitations of performing a Western blot analysis of transiently transfected cells? Can you trust these blots? Does this analysis need to be confirmed by other means or WB of stably...

01 January 2020 4,000 3 View