I want to extract DNA of unknown soil bacteria. I would like to send it back to company for sequencing. After Gell running, I would send the company or after extracting DNA, I will do Pcr than PCR product will be sent back to company.
I´ve used: Ultra Clean TM Soil DNA Isolation Kit (MO BIO Laboratories, Carlsbad, USA).
You can use any soil or stool DNA purification kit. Soil or stool kits are optimized for removal of enzymatic inhibitors from the samples. However kits based on bead beating based method are more efficient in homogenization, lysis and extraction of microbial DNA. You can check if the HiMedia soil kit is available in your country. Its cheap, efficient and yields good quality DNA for downstream applications.
http://www.himedialabs.com/intl/en/products/Molecular-Biology/Nucleic-Acid-Purification-Kit-DNA-Isolation-Kits-Column-Based-Kits/HiPurA%E2%84%A2-Soil-DNA-Purification-Kit-MB542
SD
Please see this paper
https://www.researchgate.net/publication/313846921_The_choice_of_the_DNA_extraction_method_may_influence_the_outcome_of_the_soil_microbial_community_structure_analysis
Article The choice of the DNA extraction method may influence the ou...
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