Is there any good tool to objectively measure cell confluence from a image?

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Juergen Gindlhuber

Hi,

calculating confluency can be done using image analysis tools like FIJI.

Just take an image (preferably in phase contrast) , maybe smooth it, threshold and you get the area occupied by the cells.

This process can easily be automated and will take no time at all.

Good Luck

Jürgen

Juefei Chen

Thank you Juergen, this is very useful for me.