Is there any glycoshpingolipid detection technique based on HPLC or UV?

I want to detect glycosphingolipid by UV detector in HPLC. Please, anyone let me know.

10 November 2018 9,464 2 View

What are dose and exposure time for cancer radiation therapy? Please, give some ideas or papers. Thanks?

Cancer radiation therapy.

08 September 2018 8,079 6 View

How can I measure Glucosylceramide or lactosylceramide by HPLC or spectrometer?

I want to measure glucosylceramide or lactosylceramide quantity by spectrometer or HPLC.

03 April 2018 2,002 4 View

How can I get a gene ontology software for free?

I need to download gene ontology software for free. Thanks

02 March 2018 3,102 0 View

Why double band appeared on TLC plate?

I run standard mixture on TLC plate. All standards showed with double band.

01 February 2018 3,141 4 View

What is the differences between gene silencing by shRNA and miRNA? What are procedural and theoretical differences?

I want to know advantages and disadvantages of gene silencing by shRNA and miRNA?

01 February 2018 2,643 3 View

How can I download protein structure having mutation hotspot mark?

I want to download p53 protein structure with hotspot mutation mark from protein data bank. But I could not. Would you suggest me how to use PDB or other website to see protein structure? Thanks

31 December 2017 3,639 1 View

How much sample (in amount) should be loaded on TLC plate?

I am loading 10 microliter sample slution on TLC plate. I want to know the amount of sample should be loaded on plated for detection. Thanks.

31 December 2017 2,895 7 View

How I can separate glycosphingolipids from total cell lipid?

I am trying to extract glycosphingolipid from total cellular lipids. Later I will detect shingolipid by HPLC. Thanks

07 August 2017 6,108 4 View

Could you tell me cancer resistance cells which express Glucosylceramide synthase.

Glucosylceramide synthase (GCS) performs ceramide glycosylation which is important for resistance to chemotherapy. Which cancer resistance cells express more GCS. Thanks

07 August 2017 9,076 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

How to arrange coordinates and extract climate dataset out of MPI-ESM-MR netcdf files?

Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...

28 February 2021 912 3 View