Does mRNA translation occur in red blood cell?

Red blood cell is a kind of nucleus free cell, theoretically there should not be mRNA transcription happened in the cytoplasma. But given that the red blood cells can survive in circulation for a...

06 July 2017 1,831 7 View

Does transplantation rejection happen between different subtypes of HLA-A2?

There are several subtypes of HLA-A2, such as A0201, A0202, A0203... I wonder if somebody may know if the transplantation rejection may happen among these subtypes? For example, transplant...

01 February 2016 2,279 1 View

Is there anyone familiar with the internal carotid artery injection on mice?

04 May 2015 6,053 4 View

How can I separate 4T1 cells from mouse host cells?

4T1/Balbc model is an typical model for breast cancer, but 4T1 cells lack of specific markers for flow sorting. Is there any way I can distinguish the 4T1 cells from the mouse host cells? Thank...

03 April 2015 2,427 4 View

Is there anyway to culture MDSCs sorted from tumor bearing mouse in vitro for a couple of days?

Recently I have some studies on Myeloid derived suppressor cells (MDSC). In my opinion, they mostly look like the granulocytes and monocytes. Firstly, how can I distinguish MDSC from common...

08 September 2014 7,783 5 View

What's the marker for athymic nude mice cells in flow cytometry?

How can I label the mouse cells? I have some mice with human tumor xenograft, and need to distinguish the human tumor cells and mouse cells by flow cytometry. I can label the human cells by HLA...

04 May 2014 3,646 6 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

What is the best way to fix plasma derived miRNA for subsequent cDNA synthesis?

I need to quantify blood plasma derived miRNA through q-RT-PCR. For this I need to fix RNA content in my cDNA reaction. What is the best way to do this? Currently, I take readings of my plasma...

03 February 2021 9,330 3 View

What is the general miRNA yield one gets from the Qiagen miRNEASY serum/plasma advanced kit?

I am using the Qiagen miRNEASY serum/plasma advanced kit, using 400ul of frozen plasma. What is the yield you get on average? I am using nanodrop currently for quantification. I am getting...

02 February 2021 8,806 2 View

Which miRNA arm is a right target for analysis, 3p or 5p?

I'm working on selecting mouse miRNAs to analyze and stumbling upon one confusing issue, some of the papers do not specify which arm they analyzed and they just state something like " miR-34a was...

16 January 2021 3,792 4 View

Is negative controls necessary for western blotting?

I am trying to confirm my miRNA target by western blotting. I have a ds mimic and a ss inhibitor of my miRNA. Is it necessary to use negative controls for mimic and inhibitor? If yes, how can I...

09 January 2021 2,320 3 View

Can I predict miRNA by knowing the mRNA target?

Hello! I am trying to predict in silico the miRNA by knowing the target. Most of published research starts by knowing the miRNA and predicts the target using online tools. Are you familiar with...

31 December 2020 7,433 5 View

What are the possible drawbacks of using miRNA sponge to enhance the specificity of gene expression?

I am searching for miRNA repeats put between WPRE and 3'LTR of my lentivirus, in order to enhance the specificity of transgene expression in human neuron cells. But if clinically translation of...

26 December 2020 9,921 2 View