Is there any FRET pair in existence which has excitation range higher than 500nm and can be used in living cells?

More Naincy Chandan's questions See All

Adding isopropanol directly to eluted RNA samples?

I have my RNA samples eluted in nuclease-free water. But now I want to store it for a longer period. Can I add isopropanol directly to my samples to store it without doing a second round of TriZol...

23 June 2024 2,576 4 View

Why RNA pellets of testis samples become gel during RNA isolation ?

I have been trying to isolate RNA from magur fish testis, at the end of RNA isolation, RNA pellets becoming gel when DEPC water is added, what could be the possible reason for that? What is the...

05 May 2024 5,278 1 View

How many days you take to write a research paper or commentary in your subject ? How long it can take ?

Basic Science scholars do experiments. In Economics, it takes time. Sociology or Anthropologist conduct field work. Political Science write notes. Short papers are easy to write.

08 February 2024 4,481 2 View

How could I avoid gel formation during polyurethane synthesis?

I am a postdoctoral Research Scholar from National Institute of Material Advances (NIMA), Pittsburg State University, USA. Currently I am trying to synthesis a Polyurethane acrylate oligomer from...

02 February 2024 9,460 2 View

What would you like to become in your next life ? Same person or other Identity ?

Are you happy with this present life ? Do you have any regret ? Have you accomplished everything ? Are you satisfied ? Is there anything which you could not do ? Do you think you have made life of...

24 January 2024 9,644 3 View

How can I obtain SINR, PRB allocation, TBS size and PHY rate in a 5G simulation?

I am running a 5G simulation scenario in NetSim with multiple gNBs and multiple UEs. I wish to know, for each UE, the: received SNR and SINR MCS or PHY data rate number of PRBs required and...

04 October 2023 9,858 2 View

Why Sugarcane is given FRP instead of MSP?

20 September 2023 4,257 1 View

Hoe to remove brown color from soil DNA ?

I got DNA as 500 to 2300 ng/ul with 1.5 260/280 ratio but still the color of the DNA is brown , how to remove this brown color?? I need this DNA for NGS analysis whether this is good for NGS...

11 July 2023 7,951 2 View

Could I infer that all samples were true negatives if I had no positive controls for mcr6-9 and obtained negative results?

I didn't use any positive controls for mcr6-9 in conventional PCR because they weren't available to me, and the findings for mcr6-9 were negative. Despite the fact that these genes are not common...

04 May 2023 919 5 View

Does a higher Tan delta value for a vsico-elastic polymer lead to an increased cold creep?

01 May 2023 2,014 3 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

Are these cassettes suitable for expressing PETase mutant in E. coli?

I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...

28 July 2024 7,559 1 View

Problems with ion exchange chromatography?

I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...

27 July 2024 6,605 0 View