I have been trying to isolate RNA from magur fish testis, at the end of RNA isolation, RNA pellets becoming gel when DEPC water is added, what could be the possible reason for that? What is the solution?
It is most likely the result of impurities like salts, proteins, or other organic components co-precipitating with the RNA during the isolation process that causes RNA pellets from testis samples to become gel-like during the isolation process. These impurities may prevent the RNA pellet from dissolving in water or RNA resuspension buffer, resulting in the production of a gel-like material rather than a transparent RNA solution.