How to BLAST thousands of microbiome sequences?

I'm an undergraduate student completing a capstone project working to characterize a plant microbiome. I have a couple thousand fungal ITS sequences in a fasta file. Is there any way to analyze...

03 November 2019 6,622 3 View

How to get a physiological pH when making 16% Paraformaldehyde?

I made 16% paraformaldehyde from scratch (dissolving powder in ddH20). While I was attempting to pH it, it would rapidly switch from alkaline to acidic according to the pH strips we use (range pH...

08 April 2019 6,179 3 View

How can I find out which proteins possess a specific domain or motif?

10 December 2018 8,739 2 View

What are the atomic parts of learning a character based language?

I am interested in making a suite of Unity Engine video games for second language acquisition in character based languages. The language of peak interest is Mandarin Chinese. Currently, I'm aware...

29 May 2018 6,880 1 View

Is it possible to thaw an OCT-embedded brain in order to drop-fix in PFA?

27 November 2016 9,411 1 View

Are there psychometric test (i.e. PAI, MMPI-2 RF) that have been "normed" for on-duty police officers for determing mental conditions post trauma?

02 October 2016 522 3 View

To detect Granzyme B intracellular, why the cells needed to be stimulated before staining?

if the detected cellss were stimulated in vivo, the cellls in vitro still needed to be stimulated by specific cytokines? if needed, how long is suitable for stimulating?

20 April 2016 3,502 3 View

Has anyone noticed poor agreement between replicates using the qiagen one-step RT-PCR kit?

I have been using the kit above for some time now, which performs cDNA conversion and normal qPCR in the same tube. I am noticing that despite using recently calibrated pipettes and preparing my...

30 April 2015 5,024 10 View

Why does my transformation not yield colonies?

21 April 2015 4,110 8 View

What is the best method of gDNA extraction of bacteria in semen samples?

I am trying to perform gDNA extractions on semen samples targeting Mycoplasma. I am currently using the Biosprint 96 One-For-All Vet kit however am getting very poor limit of detection in spiked...

16 March 2015 162 6 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Is anyone else having a similar situation?

I'm looking for the genetic sequence for the TAR region of HIV. If I put Transactivator Response Region, and HIV, and "genetic sequence: into a browser...is it not unreasonable to expect to be...

10 February 2021 658 5 View

Theoretical computer virology ? is still alive ?

HI Everyone! I am asking to know by you, expert in cybersecurity and mathematics, if Computer virology (the one of Cohen and Andler) is still an active field of research. That research made in...

08 February 2021 7,613 6 View

Is there no general protocol for vaccination study?

Hi, I searched vaccination study to know which MLD50 is suitable for influenza virus study in pubmed. But why every vaccination and challenge conditions are different? Is there no guideline or...

16 January 2021 7,984 3 View

Serum causes Hemagglutination in HI test. Virology Expert Please help how to resolve this issue while doing HI test for NDV and Avian Influenza?

I am doing HI Test for NDV and Avian Influenza Viruses but before going to start I first tested the serum whether any reactivity exists or not with 1% washed Chicken RBC. but I found that some...

11 January 2021 1,043 7 View

Can you trust vaccines that were made in a year, will help fight Sars-Cov2?

That vaccines have been produced in such a short time, makes me doubt if they will help fight this pandemic and if they will not do us more harm.

06 January 2021 3,469 19 View

Any papers discussing the effect of Zika virus on neuron development and its effects on neuronal functions?

I am looking into this research, any interesting papers relating to this would be amazing. Papers on pregnant women and infants would be great. Thank you :)

30 December 2020 1,962 3 View

Will the new COVID-19 strain, identified in England, affect the vaccine?

According to the CDC, the UK variant has an unusually large number of mutations -- 14 changes and 3 deletions in its genetic code that impact the building blocks of its proteins. This virus has 17...

23 December 2020 375 15 View

Can HIV drugs will be a provisional or recurring option against COVID-19?

Can HIV drugs will be a provisional or recurring option against COVID-19?

22 December 2020 7,872 2 View