How to BLAST thousands of microbiome sequences?

I'm an undergraduate student completing a capstone project working to characterize a plant microbiome. I have a couple thousand fungal ITS sequences in a fasta file. Is there any way to analyze...

03 November 2019 6,622 3 View

How to get a physiological pH when making 16% Paraformaldehyde?

I made 16% paraformaldehyde from scratch (dissolving powder in ddH20). While I was attempting to pH it, it would rapidly switch from alkaline to acidic according to the pH strips we use (range pH...

08 April 2019 6,179 3 View

How can I find out which proteins possess a specific domain or motif?

10 December 2018 8,739 2 View

What are the atomic parts of learning a character based language?

I am interested in making a suite of Unity Engine video games for second language acquisition in character based languages. The language of peak interest is Mandarin Chinese. Currently, I'm aware...

29 May 2018 6,880 1 View

Is toxicity to breast tumor cells with transfection or infection specific to RFP overexpression or is there a better red vector to use?

Our lab has been trying to generate an RFP labeled MDA-MB-231 breast cancer cell line. RFP is pretty toxic to the cells and generates abnormal clones, and they proliferate much slower than...

04 February 2018 2,738 1 View

Is it possible to thaw an OCT-embedded brain in order to drop-fix in PFA?

27 November 2016 9,411 1 View

Are there psychometric test (i.e. PAI, MMPI-2 RF) that have been "normed" for on-duty police officers for determing mental conditions post trauma?

02 October 2016 522 3 View

To detect Granzyme B intracellular, why the cells needed to be stimulated before staining?

if the detected cellss were stimulated in vivo, the cellls in vitro still needed to be stimulated by specific cytokines? if needed, how long is suitable for stimulating?

20 April 2016 3,502 3 View

Has anyone noticed poor agreement between replicates using the qiagen one-step RT-PCR kit?

I have been using the kit above for some time now, which performs cDNA conversion and normal qPCR in the same tube. I am noticing that despite using recently calibrated pipettes and preparing my...

30 April 2015 5,024 10 View

Why does my transformation not yield colonies?

21 April 2015 4,110 8 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

Can RNAscope be used to quantify the mRNA expression level of certain genes?

Hello, all, I want to explore the mRNA expression level of certain genes in the brain under physiological and pathological conditions. In previous studies, the researchers usually isolated the...

18 February 2021 1,518 1 View

How can I prevent bubbles from forming under a coverslip?

My colleague and I are having some trouble mounting 70 micrometer mouse brain slices. When we initially position the coverslip over the mounted slices, there are no bubbles but when we come back...

10 February 2021 3,580 1 View

Does anyone have a stack of images of action potentials or abnormal brain scans?

I need a stack of black and white .jpeg/.tiff/.jpg/.png images across time of either action potentials, neurons firing, or brain scans (comparing disease and normal brain, disease progression,...

09 February 2021 2,648 3 View

How do I fix tissue rolling while cutting frozen rat brain section with a cryostat?

I am cutting rat brain sections at 20um at -20C. The tissue is cutting nicely out of the O.C.T solution, but I can not get a decent section to adhere to the slide. I have included an image.

09 February 2021 1,345 5 View