What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Sample Size for Quasi-Experimental Quants using one-tailed t-test?

Hi, sorry I'm stumped. I'm going to do a study on the leadership styles (using the MLQ Form 5X) of minority women administrators in medical schools. How will I calculate the sample size? Thank...

30 January 2021 2,865 4 View

Prediction of novel precursor miRNAs in viral genomes?

Dear, I performed a multi-sequence alignment (MSA) with all the complete genome sequences of SARS-related viruses. I then used RNALalifold from the ViennaRNA package, with default parameters, to...

06 December 2020 1,704 3 View

Can I calculate mean and confidence intervals of d-prime values?

Hi everybody. I ran an experiment on memory and calculated the percentage of hits as usually reported in the literature. However, the reviewer asked me to transform my hits into d-prime values. I...

21 October 2020 3,859 1 View

Discrepancy between RNA and protein levels in single-cell seq? CD3 mRNA vs protein?

Hi all, A very quick question. I have sorted human T cells from blood using anti-CD3E, CD4 and CD8a antibodies. We did single cell seq on those sorted cells. Analysing the results, I do not see...

19 September 2020 4,953 3 View

What are the nuclei mean number from freeze tissue nuclei isolation protocol?

Hello, everyone! I'm going to start a project with single-cell ATAC-seq for analysis of open chromatin regions from freeze tissue samples. I'm lost about the nuclei concentration. If I made a...

19 June 2020 7,348 2 View

How do I compute for the Pr(Y=1|X=1) H1 and Pr(Y=1|X1) H0 for a priori estimation of required sample size?

I am trying to estimate the required sample size replicate a study which used a probit regression analysis. The paper reported the probit regression results and the marginal effects but I can't...

17 May 2020 4,826 1 View

How do you deal with your enironmental chemistry data?

Hi everybody! I am PhD in analytical chemistry and I make part of a multidisciplinary team working on the development of a web based solution focusing mainly on treatment and management of...

12 March 2020 5,597 5 View

488 laser emission crosstalk?

Image shows green fluorescence but no green fluorochrome was used in this specimen (HEK 293 cells), only Hoechst (350 nm absorption). Lasers used: 350 (upper right), 488 (upper left) and 568...

10 March 2020 7,321 3 View

Could I use a BAM file output to make a alignment with others FASTA references?

I am wondering about to use a BAM file output as a input to make a alignment to others FASTA reference genome. I have been reading how to get a FASTA formand from my BAM file but I am not sure if...

23 February 2020 9,672 5 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

How to improve and implement WBAN MAC and Radio modules under Castalia 3.2 ?

Hello researchers, Hello Castalia3.2 researchers I am a beginner with Castalia, I work on optimization of RFID systems used in wireless body networks. I simulated a WBAN with 10 nodes and one...

28 February 2021 400 1 View

How could I take into consideration the weights of observation in a system of seemingly unrelated regressions (SUR) in rstudio?

I build a model of 4 regression equations (probit and OLS) in R studio. I need to take the weights of observations into consideration. While it is simple to do so for every regression equation,...

27 February 2021 1,233 1 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View