How to BLAST thousands of microbiome sequences?

I'm an undergraduate student completing a capstone project working to characterize a plant microbiome. I have a couple thousand fungal ITS sequences in a fasta file. Is there any way to analyze...

03 November 2019 6,622 3 View

How to get a physiological pH when making 16% Paraformaldehyde?

I made 16% paraformaldehyde from scratch (dissolving powder in ddH20). While I was attempting to pH it, it would rapidly switch from alkaline to acidic according to the pH strips we use (range pH...

08 April 2019 6,179 3 View

How can I find out which proteins possess a specific domain or motif?

10 December 2018 8,739 2 View

Optimum conditions for gel electrophoresis of DNA fragment that 3.3kb long?

I want to know what would be best to use for gel electrophoresis of a 3.3kb DNA fragment. I would like to perform a gel extraction and cloning with the fragment. What would be the best voltage...

06 December 2018 3,754 1 View

What are the atomic parts of learning a character based language?

I am interested in making a suite of Unity Engine video games for second language acquisition in character based languages. The language of peak interest is Mandarin Chinese. Currently, I'm aware...

29 May 2018 6,880 1 View

How can I measure the surface coverage of carbon based nanoparticles on ITO glass?

I am working on the nanopatterning of carbon island deposited on ITO glass substrate and I want to measure the surface area of the glass that is covered by the carbon nanoparticle (number of...

24 September 2017 2,142 3 View

How to optimize solubility of my heterologous protein overexpressed at 0.04mM of IPTG and 26C incubation ?

Good morning/afternoon I am over-expressing a bacterial protein (dimer) in BL21 E. coli, which it works even with 0.04mM of IPTG, incubated at 26C. However, when I want to check the solubility of...

20 August 2017 2,306 5 View

I need the summary of Mattew finkbeiner and his method?

Subliminal priming with nearly perfect performance in the ... Matthew Finkbeiner summary of it and method

17 February 2017 2,388 1 View

Is it possible to thaw an OCT-embedded brain in order to drop-fix in PFA?

27 November 2016 9,411 1 View

Are there psychometric test (i.e. PAI, MMPI-2 RF) that have been "normed" for on-duty police officers for determing mental conditions post trauma?

02 October 2016 522 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

How to highlight novelty in your research paper?

I have come up with a method and had written a paper on it. Paper got rejected by a journal stating that there is no theoretical novelty. I am kind of confused on why the novelty that I am able to...

28 February 2021 2,393 8 View

Does the circuit modeling for planar monopole antennas using Foster canonical forms give good approximation?

Hi researchers, I am searching for some accurate way to do the circuit modeling of planar CPW-fed monopole antennas. I have gone through the literature where mostly circuit modeling is done using...

28 February 2021 7,016 1 View

What is the value of Conference Review Paper?

How much value does a review paper carry in a higher study aspirant student's profile which is presented in a Peer Reviewed Scopus Indexed conference?

27 February 2021 2,068 3 View

Centrality measures computation in Biblioshiny/ Vosviewer?

26 February 2021 7,279 2 View

Why cannot world and community leaders be gentle? Where did ethics of care go?

The whole world is in a mess and paused. We all are tired of seeing hypermasculinized and destructive leaderships all around us. This world has seen enough of the aggressive patriarchal social...

23 February 2021 292 7 View

How was your experience with Biomedical and Biotechnology Journal?

How was your publication experience with BBRJ in terms of peer review and reviewer comments. Did you find it fair and transparent ?

23 February 2021 3,810 6 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View

What is the most prevalent drug related problem in the developed countries?

Detecting the drug related problems is an important step in the pharmaceutical care plan

21 February 2021 5,421 3 View

How to calculate ultraviolet dose in Gy or J/kg?

Currently I'm doing a literature review regarding the comparisons of different types of radiation such as uv, electron beam and gamma radiation. But I'm having a difficult time converting uv unit...

11 February 2021 7,642 3 View