* Crotonic acid assay is the most rapid and simple method of quantification of PHB.
* Karr et al., 1983 showed extension of crotonic acid assay into HPLC for PHB detection.
* While Del Don et al., 1994 showed PHB, PHV detection using HPLC through alkaline digestion Article Biomass composition and methods for the determination of met...
* Both are utilizing ion exchange 'H' columns.
* Similarly is there any direct quantification of PHB, PHV using HPLC with C-18 Reverse Phase column without extensive sample pretreatment (not GC quantification)?
This paper may be of interest:
Padovani G, Emiliani G, Giovanelli A, Traversi ML, Carlozzi P. Assessment of glycerol usage by five different purple non-sulfur bacterial strains for bioplastic production. Journal of Environmental Chemical Engineering 6 (2018) 616–622. https://doi.org/10.1016/j.jece.2017.12.050
Please look at the following below links which may help you in your analysis:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2168675/
https://www.tandfonline.com/doi/abs/10.1080/01483919008049116
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