Is there any direct HPLC method for quantification of PHB,PHV using C-18 Reverse Phase column?

* Crotonic acid assay is the most rapid and simple method of quantification of PHB. * Karr et al., 1983 showed extension of crotonic acid assay into HPLC for PHB detection. * While Del Don et...

31 December 2019 2,816 2 View

How to make Standard curve for Crotonic acid assay and use it for estimation of Polyhydroxyalcanote from activated sludge?

Hello all, I have been trying to make standard curve for crotonic acid assay from polyhydroxybutyrate-co-valerate (PHBV) dissolved in chloroform. Am facing several issue regarding...

02 March 2019 8,384 1 View

GC is producing no peaks or sometime abnormal peaks of VFA in water. I used to get good quality peaks initially. What could be possible reason?

05 June 2017 2,118 2 View

What kind of filter paper is ideal for volatile suspended solid analysis? and pore size 0.45-2 micron?

09 October 2016 475 0 View

Can anyone give me an idea regarding use of aqueous sample in nonpolar/polar columns in GC for fatty acid analysis?

07 August 2016 9,396 8 View

Sensitivity of Montegomery Method for Acetic acid estimation for Concentrations below 100ppm?

I was able to construct a standard curve for acetic acid using above-mentioned method for 200-1000ppm. But I failed many a time for the same for concentrations 20-100ppm. After the entire reaction...

07 August 2016 4,580 1 View

Is it possibile to maintain an anaerobic mix culture using defined media?

An Anaerobic sludge from digester consists of various anaerobic organism. Is it possible to use media like Nutrient broth, LB broth, MSM,any such basal minimum media to maintain uniform mix...

05 June 2016 3,590 5 View

Physics: Why do we describe light by referring to its wavelength, but microwaves and radar pulses by referring to their frequency?

In physics, the description of the properties of light generally refers to its wavelength (nm), while in the range of microwaves and radio waves we refer to the frequency of a signal (e.g. kHz or...

03 March 2021 1,682 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View