Is there any direct HPLC method for quantification of PHB,PHV using C-18 Reverse Phase column?

* Crotonic acid assay is the most rapid and simple method of quantification of PHB. * Karr et al., 1983 showed extension of crotonic acid assay into HPLC for PHB detection. * While Del Don et...

31 December 2019 2,816 2 View

How to make Standard curve for Crotonic acid assay and use it for estimation of Polyhydroxyalcanote from activated sludge?

Hello all, I have been trying to make standard curve for crotonic acid assay from polyhydroxybutyrate-co-valerate (PHBV) dissolved in chloroform. Am facing several issue regarding...

02 March 2019 8,384 1 View

How to determine polyhydroxybutyrate and polyhydroxyvalerate quantitatively through crotonic acid assay?

I found a procedure for quantitative analysis of PHB and PHV using crotonic acid assay at 235nm and 285nm respectively. But scanning the standard I was unable to get a peak at 285nm even though...

01 February 2018 1,090 0 View

GC is producing no peaks or sometime abnormal peaks of VFA in water. I used to get good quality peaks initially. What could be possible reason?

05 June 2017 2,118 2 View

Can anyone give me an idea regarding use of aqueous sample in nonpolar/polar columns in GC for fatty acid analysis?

07 August 2016 9,396 8 View

Sensitivity of Montegomery Method for Acetic acid estimation for Concentrations below 100ppm?

I was able to construct a standard curve for acetic acid using above-mentioned method for 200-1000ppm. But I failed many a time for the same for concentrations 20-100ppm. After the entire reaction...

07 August 2016 4,580 1 View

Is it possibile to maintain an anaerobic mix culture using defined media?

An Anaerobic sludge from digester consists of various anaerobic organism. Is it possible to use media like Nutrient broth, LB broth, MSM,any such basal minimum media to maintain uniform mix...

05 June 2016 3,590 5 View

What thickness need for ITO to use as conductive layer?

I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...

01 March 2021 395 3 View

Glass fiber content in a gypsum board ?

Any idea on how to get the volumic ratio of glass fiber in a gypsum board ? Thank you for your help !

01 March 2021 2,501 3 View

Concrete compressive strength databases?

I am looking for datasets similar to this one: http://archive.ics.uci.edu/ml/datasets/Concrete+Compressive+Strength I am looking for concretes with different types of aggregates (Recycled,...

27 February 2021 6,801 3 View

How to properly calculate the fluid solid heat transfer between polypropylene matrix and glass fiber in a porous medium?

I'm building a cfx model of thermoplastic pultrusion process on ansys and i have a doubt on how is the proper way to set this parameter in a porous medium

24 February 2021 1,915 1 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View

How to properly calculate Interfacial area density in a porous domain?which are possible value for glass fiber?

I'm doing a cfx simulation of a multiphase flow in pultrusion and i set the Interfacial Area density in the heat exchange of a porous medium in this way IAD=Total fiber lateral surface of fiber...

23 February 2021 6,043 1 View

How can I measure the thickness of thin layers deposited on textured glass susbtrates?

I want to measure the thickness of aluminum oxide and titanium oxide layers deposited on textured glass substates. The layer thicknesses are typically below 100nm.

21 February 2021 5,465 4 View

How can I measure the surface interaction or surface interfacial chemistry between polishing abrasives and upper surface of substrate ?

I am working on polishing abrasives. I need to do few characterizations ? which can provide the on time surface chemistry of substrate with polishing abrasive during optical polishing. I would...

18 February 2021 5,319 3 View

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The regenerated cellulose film was prepared by zinc chloride/H2O system. The zinc chloride had been washed away in the regeneration process, and the wet film which was not dried had uniform...

18 February 2021 9,538 1 View

Which is the best silane for making a borosilicate glass surface hydrophobic permanently? and what is the protocol?

Regarding my needs I am looking for a glass coating agent that makes the glass hydrophobic permanently

09 February 2021 3,924 5 View